Comparison of Bayer Advia Centaur immunoassay results obtained on samples collected in four different Becton Dickinson Vacutainer tubes.

Author(s): Morovat A, James TS, Cox SD, Norris SG, Rees MC, Gales MA, Taylor RP

Publication: Ann Clin Biochem, 2006, Vol. 43, Page 481-7

PubMed ID: 17132279 PubMed Review Paper? No

Purpose of Paper

Conclusion of Paper

Studies

1. Study Purpose

   The purpose of this study was to determine the effects of blood collection tube type on the results of 15 immunoassays, including ones for a variety of hormones as well as CA125, CA15-3, vitamin B12, and folate, performed by the Bayer Advia Centaur analyzer.

   Summary of Findings:

   Results were comparable between specimens collected in plain plastic Vacutainers, old SST II Advance (discontinued in October 2004), new SST II Advance, and lithium-heparin plasma from PST II for TSH, FT4, FT3, PTH, E2, and prolactin. Serum collected in new SST II Advance showed slightly increased average FSH levels (+4.0%) and decreased average vitamin B12 levels (-10.0%) compared to serum collected in the plain plastic tubes. Serum collected in old SST II Advance showed increased average cortisol levels (+11.2%) and decreased average vitamin B12 levels (-16.6%) compared to specimens collected in plain plastic tubes. Plasma collected in PST II showed increased average LH (+8.0%), FSH (+7.9%), and folate levels (+37.7%), and decreased average cortisol (-34.3%), testosterone (-22.8%), and progesterone levels (-11.8%), and also exhibited positive absolute bias for vitamin B12 and CA15-3 compared to serum collected in plain plastic tubes.

   Biospecimens

   • Bodily Fluid - Plasma
   • Bodily Fluid - Serum
   • Bodily Fluid - Blood

   Preservative Types

   • None (Fresh)

   Diagnoses:

   • Normal

   Platform:

   Analyte	Technology Platform
   Glycoprotein	Clinical chemistry/auto analyzer
   Peptide	Clinical chemistry/auto analyzer
   Small molecule	Clinical chemistry/auto analyzer
   Steroid	Clinical chemistry/auto analyzer

   Pre-analytical Factors:

   Classification	Pre-analytical Factor	Value(s)
   Biospecimen Acquisition	Type of collection container/solution	Old SST II Advance New SST II Advance PST II Plain plastic
   Biospecimen Aliquots and Components	Blood and blood products	Plasma Serum

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