The influence of different sample collection types on the levels of markers used for Down's syndrome screening as measured by the Kryptor Immunosassay system.

Author(s): Spencer K

Publication: Ann Clin Biochem, 2003, Vol. 40, Page 166-8

PubMed ID: 12662405 PubMed Review Paper? No

Purpose of Paper

Conclusion of Paper

Studies

1. Study Purpose

   The purpose of this study was to determine whether plasma could be used instead of maternal serum for measurement of AFP, PAPP-A, and free beta-hCG during prenatal screening for Downs syndrome. All specimens were refrigerated.

   Summary of Findings:

   Free beta-hCG measurements were not significantly different between serum and heparinized, citrated or EDTA-plasma. However, AFP levels were significantly reduced in citrated and EDTA-plasma compared to serum levels. Furthermore, significantly lower PAPP-A values were obtained from plasma collected with any of the three anticoagulants when compared to levels obtained from serum suggesting that serum must be used for measurement of these three maternal biochemical markers of Downs syndrome.

   Biospecimens

   • Bodily Fluid - Plasma
   • Bodily Fluid - Serum
   • Bodily Fluid - Blood

   Preservative Types

   • Other Preservative

   Diagnoses:

   • Pregnant

   Platform:

   Analyte	Technology Platform
   Protein	Hematology/ auto analyzer
   Glycoprotein	Hematology/ auto analyzer

   Pre-analytical Factors:

   Classification	Pre-analytical Factor	Value(s)
   Biospecimen Acquisition	Anticoagulant	Lithium heparin Sodium citrate None Potassium EDTA
   Biospecimen Aliquots and Components	Blood and blood products	Plasma Serum

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