NIH, National Cancer Institute, Division of Cancer Treatment and Diagnosis (DCTD) NIH - National Institutes of Health National Cancer Institute DCTD - Division of Cancer Treatment and Diagnosis

An appraisal of the "Vacutainer" system for blood collection.

Author(s): Ames AC, Bamford E

Publication: Ann Clin Biochem, 1975, Vol. 12, Page 151-5

PubMed ID: 15637911 PubMed Review Paper? No

Purpose of Paper

The purpose of this paper was to determine the effects of blood collection container and method on the incidence of hemolysis and chloride and bicarbonate concentrations in serum.

Conclusion of Paper

While 40/97 specimens (41%) obtained using a conventional syringe and transferred to a tube showed macroscopic hemolysis, none of the 107 specimens collected in a Vacutainer showed macroscopic levels of hemolysis. There were no significant differences in serum chloride or serum bicarbonate concentrations between specimens collected using a conventional syringe technique and those collected in Vacutainers. The authors state that the use of plastic syringes and containers for blood collection resulted in frequent auto analyzer blockages due to fine fibrin clots, while this did not occur when glass Vacutainers were used.

Studies

  1. Study Purpose

    The purpose of this study was to determine the effects of blood collection container and method on the incidence of hemolysis and chloride and bicarbonate concentrations in serum.

    Summary of Findings:

    While 40/97 specimens (41%) obtained using a conventional syringe and transferred to a tube showed macroscopic hemolysis, none of the 107 specimens collected in a Vacutainer showed macroscopic levels of hemolysis. There were no significant differences in serum chloride or serum bicarbonate concentrations between specimens collected using a conventional syringe technique and those collected in Vacutainers. The authors state that the use of plastic syringes and containers for blood collection resulted in frequent auto analyzer blockages due to fine fibrin clots, while this did not occur when glass Vacutainers were used.

    Biospecimens
    Preservative Types
    • None (Fresh)
    Diagnoses:
    • Not specified
    Platform:
    AnalyteTechnology Platform
    Small molecule Clinical chemistry/auto analyzer
    Electrolyte/Metal Clinical chemistry/auto analyzer
    Cell count/volume Macroscopic observation
    Pre-analytical Factors:
    ClassificationPre-analytical FactorValue(s)
    Biospecimen Acquisition Method of fluid acquisition Syringe draw
    Vacuum tube
    Biospecimen Acquisition Type of collection container/solution Plastic syringe and plastic tube
    Glass Vacutainer

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