Hypothermia and acidosis synergistically impair coagulation in human whole blood.

Author(s): Dirkmann D, Hanke AA, Görlinger K, Peters J

Publication: Anesth Analg, 2008, Vol. 106, Page 1627-32

PubMed ID: 18499589 PubMed Review Paper? No

Purpose of Paper

Conclusion of Paper

Studies

1. Study Purpose

   The purpose of this study was to determine the effects of blood pH and storage temperature on coagulation parameters in sodium citrate-whole blood. Specimens were acidified with hydrochloric acid and incubated at the relevant temperature for 30 min. Specimens were recalcified with calcium chloride.

   Summary of Findings:

   Blood analyzed at 30 degrees C had longer CT and CFT and lower AA and MCF in extrinsic and intrinsic tests than blood analyzed at 36 or 39 degrees C. When blood was analyzed at 36 degrees C, there was no effect of blood pH on the variables, but when blood was analyzed at 33 degrees C, specimens with lower pH tended to have longer CT and CFT and lower AA and MCF than specimens with a pH of 7.37 or those analyzed at 36 or 39 degrees C. In the presence of cytochalasin D, MCF was not influenced by temperature or pH. Extrinsic and intrinsic clot lysis were decreased in specimens analyzed at 30 degrees C compared to those analyzed 36 degrees C, and clot lysis increased in some specimens at 39 degrees C compared to specimens at 33 degrees C, but there were no clear effects of pH on clot lysis.

   Biospecimens

   • Bodily Fluid - Blood

   Preservative Types

   • None (Fresh)

   Diagnoses:

   • Normal

   Platform:

   Analyte	Technology Platform
   Morphology	Hematology/ auto analyzer

   Pre-analytical Factors:

   Classification	Pre-analytical Factor	Value(s)
   Storage	Storage temperature	30 degrees C 33 degrees C 36 degrees C 39 degrees C
   Biospecimen Aliquots and Components	pH	7 7.1 7.2 7.37

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