Utility of tissue microarrays for assessment of chromosomal abnormalities in chromophobe renal cell carcinoma.
Author(s): Brunelli M, Delahunt B, Ficarra V, Gobbo S, Eccher A, Cossu-Rocca P, Zattoni F, Cheng L, Eble JN, Martignoni G
Publication: Anal Quant Cytol Histol, 2009, Vol. 31, Page 401-9
PubMed ID: 20698356 PubMed Review Paper? No
Purpose of Paper
Conclusion of Paper
Studies
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Study Purpose
The purpose of this study was to compare FISH results in TMAs versus whole section slides of chromophobe renal cell carcinoma specimens. 3 cores from each of 6 archived formalin-fixed paraffin-embedded tumors were included on the TMA, and the average score of either all three cores, or of the 3 possible core pairings was calculated. All 4 average scores, as well as individual core scores, were compared to whole section scores.
Summary of Findings:
Substantial concordance (K values 0.46-0.75) to almost perfect concordance (K values 0.79-0.99) in FISH analysis of chromosomes 1, 2, 6, 10, and 17 was observed between TMA and whole sections when the average score of 3 TMA cores was compared to scores of whole sections. Concordance indices were highly variable when the score of only one or the average of two cores was used for the comparison with whole section scores.
Biospecimens
Preservative Types
- Formalin
Diagnoses:
- Neoplastic - Carcinoma
- Neoplastic - Normal Adjacent
Platform:
Analyte Technology Platform DNA FISH DNA Tissue microarray Pre-analytical Factors:
Classification Pre-analytical Factor Value(s) FISH Specific Targeted nucleic acid Centromere of chromosome 1
Centromere of chromosome 2
Centromere of chromosome 6
Centromere of chromosome 10
Centromere of chromosome 17
Biospecimen Aliquots and Components Type of slide Whole section
Tissue microarray
Biospecimen Aliquots and Components Aliquot size/volume 1 TMA core
2 TMA cores
3 TMA cores
Tissue microarray Specific Data handling Individual cores scored
Pairs of cores averaged
Three cores averaged