Cancer Diagnosis Program | Biorepositories and Biospecimen Research Branch

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Impact of warm ischemia on gene expression analysis in surgically removed biosamples.

Author(s): Ma Y, Dai H, Kong X

Publication: Anal Biochem, 2012, Vol. 423, Page 229-35

PubMed ID: 22343191 PubMed Review Paper? Yes

Purpose of Paper

Conclusion of Paper

Studies

1. Study Purpose

   This study reviewed the literature on the effects of room temperature storage of specimens on RNA integrity in eight tissue types. Data from 14 papers investigating specimens from colorectal tumor and normal tissues, lung tumors, normal liver tissue, ovarian tumors, prostate tumor and normal tissue, breast tumor and normal tissues, normal tonsil, and pancreatic tumors were included. The number of patients studied ranged from 1-82. Details of specimen handling and analysis were not specified but it is assumed they were fresh and analyzed by bioanalyzer.

   Summary of Findings:

   With the exception of colorectal tissue, RNA integrity numbers (RIN) remained above 6 when specimens were stored at room temperature for up to 5 h. While RNA integrity in colorectal tissue as determined by RIN or the ratio of 28S to 18S rRNA was affected by 90-120 min at room temperature in two studies, the other two studies found no effect of 30 min and up to 16 h storage at room temperature on the ratio of 28S to 18S rRNA. Two studies demonstrated that storage at room temperature did not affect RNA integrity in lung (up to 2 h and 5 h, respectively), breast (3 h and 24 h, respectively) and ovary (2 h and 18 h, respectively) specimens. Further, the single studies investigating the effects of room temperature storage on pancreas and prostate found no effect on RIN after 1 h and a less than 15% decline in RIN after 8 h and a decrease in the 28S/18S rRNA ratio from 1.5 to 1.4 after 16 h on liver and tonsil specimens, respectively.

   Biospecimens

   • Tissue - Breast
   • Tissue - Colorectal
   • Tissue - Lung
   • Tissue - Prostate
   • Tissue - Ovary
   • Tissue - Tonsil
   • Tissue - Pancreas
   • Tissue - Liver

   Preservative Types

   • None (Fresh)

   Diagnoses:

   • Neoplastic - Carcinoma
   • Neoplastic - Not specified
   • Normal

   Platform:

   Analyte	Technology Platform
   RNA	Automated electrophoresis/Bioanalyzer
   RNA	Electrophoresis
   RNA	Northern blot

   Pre-analytical Factors:

   Classification	Pre-analytical Factor	Value(s)
   Biospecimen Acquisition	Biospecimen location	Breast Colorectal Lung Liver Ovary Pancreas Tonsil Prostate
   Storage	Time at room temperature	0 min-48 h

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