Plasma storage at -80 degrees C does not protect matrix metalloproteinase-9 from degradation.

Author(s): Rouy D, Ernens I, Jeanty C, Wagner DR

Publication: Anal Biochem, 2005, Vol. 338, Page 294-8

PubMed ID: 15745750 PubMed Review Paper? No

Purpose of Paper

Conclusion of Paper

Studies

1. Study Purpose

   This study sought to determine whether plasma protein expression and enzymatic activity of MMP-9, a self-activable cardiovascular marker, fluctuates after storage of plasma biospecimens at -80 degrees C for 1 to 43 months.

   Summary of Findings:

   Plasma MMP-9 levels (protein levels as well as enzymatic activity) show a time-dependent decrease over time after storage at -80 degrees C. Robust decreases in MMP-9 levels were observed after as little as 2 months of storage, with levels degrading to 35% and 1% after 24 and 43 months, respectively, compared to 1 month of frozen storage. Plasma protein levels of MMP-2 and TIMP-1 did not vary as a result of storage duration. Rapid analysis or storage in liquid nitrogen was recommended by the authors to minimize time-dependent enzymatic degradation.

   Biospecimens

   • Bodily Fluid - Plasma

   Preservative Types

   • Frozen

   Diagnoses:

   • Cardiovascular Disease

   Platform:

   Analyte	Technology Platform
   Protein	ELISA
   Protein	Western blot
   Protein	Zymography

   Pre-analytical Factors:

   Classification	Pre-analytical Factor	Value(s)
   Storage	Storage duration	1 month 2 months 3 months 4-12 months 13-24 months 25-36 months 37-43 months
   Storage	Storage temperature	-80 degrees C
   ELISA Specific	Targeted peptide/protein	MMP-9 MMP-2 TIMP-1

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