A systematic error in the determination of blood PCO2.

Author(s): Hansen JE, Simmons DH

Publication: Am Rev Respir Dis, 1977, Vol. 115, Page 1061-3

PubMed ID: 45377 PubMed Review Paper? No

Purpose of Paper

Conclusion of Paper

Studies

1. Study Purpose

   The purpose of this study was to determine the effects of sodium heparin volume on pCO2, bicarbonate, base excess and pH in blood specimens. On 4 days, a single specimen of anti-coagulated venous blood was added to syringes containing various volumes of sodium heparin, mixed by hand, and iced. Specimens were warmed to 37 degrees C for analysis.

   Summary of Findings:

   Dilution of blood with sodium heparin had no effects on specimen pH, but pCO2, bicarbonate and base excess decreased proportionally with the dilution factor. The authors recommend the use of larger specimen volumes to decrease the dilution effects without increasing air exposure.

   Biospecimens

   • Bodily Fluid - Blood

   Preservative Types

   • Other Preservative

   Diagnoses:

   • Not specified

   Platform:

   Analyte	Technology Platform
   Gas	Clinical chemistry/auto analyzer
   Small molecule	pH
   Small molecule	Clinical chemistry/auto analyzer

   Pre-analytical Factors:

   Classification	Pre-analytical Factor	Value(s)
   Biospecimen Acquisition	Anticoagulant	Multiple concentrations evaluated Sodium heparin

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