Tissue microarray sampling strategy for prostate cancer biomarker analysis.

Author(s): Rubin MA, Dunn R, Strawderman M, Pienta KJ

Publication: Am J Surg Pathol, 2002, Vol. 26, Page 312-9

PubMed ID: 11859202 PubMed Review Paper? No

Purpose of Paper

Conclusion of Paper

Studies

1. Study Purpose

   The purpose of this study was to determine the optimal number of cores on a TMA to accurately represent ki-67 IHC staining and predict prostate cancer outcome. The authors record expression level based on the 90th percentile of the 10 measurements taken per sample. The ki-67 expression study included only one specimen that was divided into 10 sections from each of which 5 cores were obtained. The outcome study included 6-10 cores taken from each of 88 cases.

   Summary of Findings:

   Sampling of 3 cores was necessary to overcome the variability in ki-67 expression, but additional cores did not increase reliability of the measurement. Prediction of outcome was highly significant when 3 or more cores were stained, and while low variability in outcome predictions was observed when at least 4 cores were stained, the addition of more cores did not decrease variability.

   Biospecimens

   • Tissue - Prostate

   Preservative Types

   • Formalin

   Diagnoses:

   • Neoplastic - Carcinoma

   Platform:

   Analyte	Technology Platform
   Protein	Immunohistochemistry
   Protein	Tissue microarray

   Pre-analytical Factors:

   Classification	Pre-analytical Factor	Value(s)
   Biospecimen Aliquots and Components	Type of slide	Tissue microarray Whole tissue section
   Immunohistochemistry Specific	Targeted peptide/protein	Ki-67
   Biospecimen Aliquots and Components	Aliquot size/volume	1 core 2 cores 3 cores 4 cores 5 cores 6-10 cores

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