Changes in Differential Gene Expression because of Warm Ischemia Time of Radical Prostatectomy Specimens

Author(s): Dash A, Maine IP, Varambally S, Shen R, Chinnaiyan AM, Rubin MA

Publication: Am J Pathol, 2002, Vol. 161, Page 1743

PubMed ID: 12414521 PubMed Review Paper? No

Purpose of Paper

Conclusion of Paper

Studies

1. Study Purpose

   The purpose of this study was to determine if prolonged tissue ischemia (the time the specimen was stored at room temperature following surgical resection) altered prostate gene expression.

   Summary of Findings:

   Using microarray, 0.6% of genes (61 genes, 41 of which were named) were upregulated in prostate tissues stored at room temperature for 1 h or longer compared to 0 h controls. Genes displaying elevated expression included several early response genes (early growth response 1, EGR-1; jun B proto oncogene, junB; jun D proto oncogene, jun D; activating transcription factor 3, ATF3). The degree of upregulation was variable and ranged between minute increases to nearly 2-fold upregulation (EGR-1, junB).

   Biospecimens

   • Tissue - Prostate

   Preservative Types

   • OCT

   Diagnoses:

   • Neoplastic - Carcinoma

   Platform:

   Analyte	Technology Platform
   RNA	DNA microarray

   Pre-analytical Factors:

   Classification	Pre-analytical Factor	Value(s)
   Biospecimen Acquisition	Cold ischemia time	0 h 0.5 h 1 h 3 h 5 h
   DNA microarray Specific	Targeted nucleic acid	EGR1 junB junD ATF3

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2. Study Purpose

   The purpose of this study was to determine if ischemia-induced increases in gene expression translate to increased protein expression, specifically EGR1.

   Summary of Findings:

   Microarray analysis of EGR1 expression was confirmed by Western blot. Protein expression of EGR1 increased as a function of progressive cold ischemia time (time stored at room temperature following surgical resection) beginning after 1 h.

   Biospecimens

   • Tissue - Prostate

   Preservative Types

   • OCT

   Diagnoses:

   • Neoplastic - Carcinoma

   Platform:

   Analyte	Technology Platform
   Protein	Western blot

   Pre-analytical Factors:

   Classification	Pre-analytical Factor	Value(s)
   Biospecimen Acquisition	Cold ischemia time	0 h 0.5 h 1 h 3 h 5 h
   Western blot Specific	Targeted peptide/protein	EGR1

   View more