Cancer Diagnosis Program | Biorepositories and Biospecimen Research Branch

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Are the cytokines interleukin-6 and angiogenin stable in frozen amniotic fluid?

Author(s): Spong CY, Ghidini A, Ossandon M, Walker CN, Pezzullo JC

Publication: Am J Obstet Gynecol, 1998, Vol. 178, Page 783-6

PubMed ID: 9579444 PubMed Review Paper? No

Suggested by: ISBER

Purpose of Paper

Conclusion of Paper

Studies

1. Study Purpose

   The purpose of this study was to determine the effects of storage on angiogenin and IL-6 concentrations in amniotic fluid. Specimens were stored at -70 degrees C for up to 2 years prior to the commencement of this study.

   Summary of Findings:

   After 1 year of storage at -70 degrees C, angiogenin levels decreased from an average of 26.7 ng/mL to 12.4 ng/mL (p<0.001) with levels in individual specimens declining between 15.6% and 70.8%. The largest decreases in angiogenin concentrations were found in the specimens collected 2 years prior to the first angiogenin measurement (p=0.008). After 1 year of storage at -70 degrees C, IL-6 levels decreased from an average of 289.2 pg/mL to 230.8 pg/mL (p<0.001). IL-6 in individual specimens declined by up to 89.2%, but 2 of 30 specimens showed increase in IL-6 of up to 94.6% after storage. Initial levels of angiogenin and IL-6 had no effect on the rate of decline.

   Biospecimens

   • Bodily Fluid - Amniotic Fluid

   Preservative Types

   • Frozen

   Diagnoses:

   • Not specified
   • Other diagnoses

   Platform:

   Analyte	Technology Platform
   Protein	ELISA

   Pre-analytical Factors:

   Classification	Pre-analytical Factor	Value(s)
   Storage	Storage temperature	-70 degrees C
   Preaquisition	Diagnosis/ patient condition	Single gestation Intrauterine growth restriction Pre-term delivery
   Storage	Storage duration	0 years 1 year

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