NIH, National Cancer Institute, Division of Cancer Treatment and Diagnosis (DCTD) NIH - National Institutes of Health National Cancer Institute DCTD - Division of Cancer Treatment and Diagnosis

Variation in serum and plasma PTH levels in second-generation assays in hemodialysis patients: a cross-sectional study.

Author(s): Joly D, Drueke TB, Alberti C, Houillier P, Lawson-Body E, Martin KJ, Massart C, Moe SM, Monge M, Souberbielle JC

Publication: Am J Kidney Dis, 2008, Vol. 51, Page 987-95

PubMed ID: 18430500 PubMed Review Paper? No

Purpose of Paper

The purpose of this paper was to determine the effects of anticoagulant, assay type and storage on the measurement of PTH in blood specimens.

Conclusion of Paper

While storage of specimens at room temperature had no significant effect on the measurement of PTH, citrated plasma consistently had lower levels of PTH than EDTA plasma and serum. Most of the variability was interindividual, but 2.5% of the variability was attributed to kit type and 0.7% to anticoagulant. The differences in anticoagulant and kit type led to different Kidney Disease Outcomes Quality Initiative (KDOQI) classifications.

Studies

  1. Study Purpose

    The purpose of this study was to determine the effects of anticoagulant, assay type and storage on the measurement of PTH in blood specimens.

    Summary of Findings:

    Anticoagulant and assay type significantly affected the measured PTH variability (both p<0.001), but storage did not. While interindividual variability accounted for 96.8% of the variability, 2.5% of the variability was attributed to kit and 0.7% to anticoagulant. Citrated plasma consistently had lower levels of PTH than EDTA plasma and serum. Importantly, the differences in anticoagulant and kit type led to different Kidney Disease Outcomes Quality Initiative (KDOQI) classifications.

    Biospecimens
    Preservative Types
    • Frozen
    Diagnoses:
    • Diabetes Type 2
    • Not specified
    Platform:
    AnalyteTechnology Platform
    Peptide Clinical chemistry/auto analyzer
    Pre-analytical Factors:
    ClassificationPre-analytical FactorValue(s)
    Biospecimen Aliquots and Components Blood and blood products Plasma
    Serum
    Storage Time at room temperature 0 h
    16-22 h
    Clinical chemistry/auto analyzer Specific Technology platform Roche Elecsys
    Bayer Advia Centaur
    DiaSorin LIASON
    DPC Immulite
    Abbot Achitect
    Beckman-Coulter Access
    Biospecimen Acquisition Anticoagulant EDTA
    Sodium citrate
    None

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