Epigenomic profiling reveals DNA-methylation changes associated with major psychosis.

Author(s): Mill J, Tang T, Kaminsky Z, Khare T, Yazdanpanah S, Bouchard L, Jia P, Assadzadeh A, Flanagan J, Schumacher A, Wang SC, Petronis A

Publication: Am J Hum Genet, 2008, Vol. 82, Page 696-711

PubMed ID: 18319075 PubMed Review Paper? No

Purpose of Paper

Conclusion of Paper

Studies

1. Study Purpose

   The purpose of this study was to determine if DNA methylation patterns in brain regions vary as a result of patient gender, postmortem interval, or disease state.

   Summary of Findings:

   DNA methylation state was not correlated to postmortem interval, brain weight, or brain pH. The extent of CpG island methylation of an area approximately 30 kb upstream of the mitogen-activated protein kinase kinase I (MEK1) gene was significant correlated and inversely proportional to prolonged use of anti-psychotic drugs in biospecimens procured from both male and females diagnosed with schizophrenia but not bipolar disorder. Several sex-specific methylation patterns were also observed.

   Biospecimens

   • Tissue - Brain

   Preservative Types

   • Frozen

   Diagnoses:

   • Bipolar Disorder
   • Schizophrenia

   Platform:

   Analyte	Technology Platform
   DNA	DNA microarray

   Pre-analytical Factors:

   Classification	Pre-analytical Factor	Value(s)
   Preaquisition	Patient gender	Female Male
   Biospecimen Acquisition	Organ measurements	Brain weight 1314-1486 g
   Preaquisition	Diagnosis/ patient condition	Bipolar disorder Schizophrenia
   Preaquisition	Other drugs	Prolonged use of antipsychotic medication (varying doses)

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