Comparative study of bedside and laboratory measurements of hemoglobin.

Author(s): Krenzischek DA, Tanseco FV

Publication: Am J Crit Care, 1996, Vol. 5, Page 427-32

PubMed ID: 8922158 PubMed Review Paper? No

Purpose of Paper

Conclusion of Paper

Studies

1. Study Purpose

   The purpose of this study was to determine the effects of blood analyzer type (portable versus laboratory), the length of time blood specimens were shaken in the syringe after collection, and the amount of blood discarded before specimen collection from a central venous or arterial catheter on hemoglobin measurements from post-operative patients. While capillary, venous, and arterial collections were all used in this study, direct comparisons between collection methods were not made. Fingerprick collections of capillary blood resulted in only enough blood for a bedside measurement and were compared with venous specimens measured in the laboratory.

   Summary of Findings:

   There were no differences in hemoglobin measurements taken at bedside with the HemoCue system and those measured in the laboratory on the Coulter STKR analyzer, regardless of blood collection method. Shaking venous or arterial blood in a syringe for 10, 30, or 60 seconds prior to measuring hemoglobin with the HemoCue system did not affect hemoglobin measurements. Discarding 1.0 mL of blood from the arterial catheter before specimen collection and 2.0 mL of blood from the central venous catheter did not significantly affect hemoglobin measurements compared to discarding more blood.

   Biospecimens

   • Bodily Fluid - Blood

   Preservative Types

   • None (Fresh)

   Diagnoses:

   • Not specified

   Platform:

   Analyte	Technology Platform
   Protein	Hematology/ auto analyzer

   Pre-analytical Factors:

   Classification	Pre-analytical Factor	Value(s)
   Biospecimen Aliquots and Components	Biospecimen mixing	10 s 30 s 60 s
   Biospecimen Aliquots and Components	Aliquot size/volume	Initial 1.0 mL discarded Initial 2.0 mL discarded Initial 2.5 mL discarded Initial 4.5 mL discarded Initial 5.0 mL discarded Initial 6.0 mL discarded Initial 10.5 mL discarded
   Hematology/ auto analyzer Specific	Technology platform	HemoCue system (bedside) Coulter STKR system (laboratory)
   Biospecimen Acquisition	Anatomical location of blood draw	Artery Vein Capillary

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