Cancer Diagnosis Program | Biorepositories and Biospecimen Research Branch

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No evidence for interference of h&e staining in DNA testing: usefulness of DNA extraction from h&e-stained archival tissue sections.

Author(s): Morikawa T, Shima K, Kuchiba A, Yamauchi M, Tanaka N, Imamura Y, Liao X, Qian ZR, Brahmandam M, Longtine JA, Lindeman NI, Fuchs CS, Ogino S

Publication: Am J Clin Pathol, 2012, Vol. 138, Page 122-9

PubMed ID: 22706867 PubMed Review Paper? No

Purpose of Paper

Conclusion of Paper

Studies

1. Study Purpose

   The purpose of this study was to determine the effects of histological staining of FFPE tissue sections on DNA integrity and analysis of DNA by real-time PCR, microsatellite analysis and pyrosequencing. Non-coverslipped whole sections of FFPE tissue were either unstained , hematoxylin stained for 3 min, eosin stained for 15 sec, or hematoxylin and eosin stained. Tissue was scraped into tubes, and DNA was extracted with QIAamp.

   Summary of Findings:

   Histological staining of FFPE tissue sections did not impact DNA integrity as determined by electrophoresis, real-time PCR GAPDH CT values, microsatellite mean peak height, and sequencing quality or results.

   Biospecimens

   • Tissue - Colorectal

   Preservative Types

   • Formalin

   Diagnoses:

   • Neoplastic - Carcinoma
   • Not specified

   Platform:

   Analyte	Technology Platform
   DNA	Electrophoresis
   DNA	DNA sequencing
   DNA	Microsatellite analysis
   DNA	Real-time qPCR

   Pre-analytical Factors:

   Classification	Pre-analytical Factor	Value(s)
   DNA sequencing Specific	Type of tissue stain	Hematoxylin Eosin H&E Unstained
   Real-time qPCR Specific	Type of tissue stain	Hematoxylin Eosin H&E Unstained
   Real-time qPCR Specific	Targeted nucleic acid	GAPDH
   Electrophoresis Specific	Type of tissue stain	Hematoxylin Eosin H&E Unstained

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