Combined core needle biopsy and fine-needle aspiration with ancillary studies correlate highly with traditional techniques in the diagnosis of nodal-based lymphoma.

Author(s): Amador-Ortiz C, Chen L, Hassan A, Frater JL, Burack R, Nguyen TT, Kreisel F

Publication: Am J Clin Pathol, 2011, Vol. 135, Page 516-24

PubMed ID: 21411774 PubMed Review Paper? No

Purpose of Paper

Conclusion of Paper

Studies

1. Study Purpose

   The purpose of this study was to determine the effects of specimen collection method and location on diagnosis of lymphoma using a panel of lymphoma markers.

   Summary of Findings:

   A diagnosis from CNB was established in 90.1% of the 237 cases. When CNB were from a superficial lymph node, diagnosis was obtained in 95% of specimens, while diagnosis was determined for 86.4% of CNB from deeply seated nodes (p=0.025). CNB were large enough specimens for IHC and flow cytometry 66.9% and 52.1% of the time, respectively, and a complete diagnosis was obtained by IHC and flow cytometry in 57% and 47% of cases, respectively. 44 cases were not diagnosable from the CNB because the specimen was too small or the results were equivocal. An excisional biopsy was performed for 15 of the cases with undiagnosed CNB allowing for a fully classified diagnosis in all cases. Complete concordance was obtained in 37 of the 38 cases for which a diagnosis was obtained from both CNB and excisional biopsy specimens. The discordant specimen was diagnosed from a CNB to be a reactive lymphoid infiltrate, but was found to be classical Hodgkin lymphoma by excisional biopsy.

   Biospecimens

   • Tissue - Lymph Node

   Preservative Types

   • Formalin

   Diagnoses:

   • Neoplastic - Lymphoma
   • Neoplastic - Benign

   Platform:

   Analyte	Technology Platform
   Protein	Flow cytometry
   Protein	Immunohistochemistry
   RNA	In situ hybridization
   DNA	FISH

   Pre-analytical Factors:

   Classification	Pre-analytical Factor	Value(s)
   Biospecimen Acquisition	Biospecimen location	Thorax Groin Cervix Axilla Retroperitoneum
   Flow cytometry Specific	Technology platform	Immunohistochemistry
   Preaquisition	Diagnosis/ patient condition	Diffuse large B-cell lymphoma T-Cell lymphoma Hodgkin lymphoma Benign Small lymphocytic lymphoma/chronic lymphocytic leukemia Mantle lymphoma Marginal lymphoma Burkitt lymphoma Follicular lymphoma
   Biospecimen Acquisition	Method of tissue acquisition	Core needle biopsy Excisional biopsy

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