Minimum specimen volume requirements for routine coagulation testing: dependence on citrate concentration.

Author(s): Adcock DM, Kressin DC, Marlar RA

Publication: Am J Clin Pathol, 1998, Vol. 109, Page 595-9

PubMed ID: 9576579 PubMed Review Paper? No

Purpose of Paper

Conclusion of Paper

Studies

1. Study Purpose

   The purpose of this study was to determine the effects of tube fill volume and citrate concentration (3.8% versus 3.2%) on PT and APTT in plasma from healthy volunteers and patients on anticoagulant therapy.

   Summary of Findings:

   In plasma from healthy volunteers, PT was significantly increased by tube fill volumes of 70% or less in 3.8% citrate tubes and fill volumes of 50% or less in 3.2% citrate tubes. APTT was significantly increased in plasma from healthy volunteers by fill volumes of 80% or less in 3.8% citrate tubes and fill volumes of 60% or less in 3.2% citrate tubes. Clinically significant increases in PT in specimens from patients on oral anticoagulant therapy were seen with fill volumes of 80% or less in the 3.8% citrate tubes and with fill volumes of 70% or less in the 3.2% citrate tubes. Clinically significant increases in APTT were seen in specimens from patients on oral anticoagulant therapy with fill volumes of 70% or less in the 3.8% citrate tubes and 60% or less in the 3.2% citrate tubes.

   Biospecimens

   • Bodily Fluid - Plasma
   • Bodily Fluid - Blood

   Preservative Types

   • None (Fresh)

   Diagnoses:

   • Normal
   • Not specified

   Platform:

   Analyte	Technology Platform
   Morphology	Hematology/ auto analyzer

   Pre-analytical Factors:

   Classification	Pre-analytical Factor	Value(s)
   Biospecimen Aliquots and Components	Aliquot size/volume	Arbitrarily underfilled (<5.0 mL) 2.0 mL 2.5 mL 3.0 mL 3.5 mL 4.0 mL 4.5 mL 5.0 mL
   Preaquisition	Other drugs	None Oral anticoagulant therapy
   Biospecimen Acquisition	Anticoagulant	Citrate Multiple concentrations evaluated

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