Effect of 3.2% vs 3.8% sodium citrate concentration on routine coagulation testing.

Author(s): Adcock DM, Kressin DC, Marlar RA

Publication: Am J Clin Pathol, 1997, Vol. 107, Page 105-10

PubMed ID: 8980376 PubMed Review Paper? No

Purpose of Paper

Conclusion of Paper

Studies

1. Study Purpose

   The purpose of this study was to determine the effects of sodium citrate concentration and assay reagent on PT and aPTT in plasma from healthy volunteers and patients on anticoagulant therapy.

   Summary of Findings:

   When the responsive reagents (Actin FS and Innovin) were used, the normal ranges of aPTT and PT increased when specimens from healthy volunteers were collected in 3.8% citrate compared to 3.2% citrate (both p<0.001). There was no significant difference in aPTT or PT ranges between specimens from healthy volunteers collected in 3.8 or 3.2% citrate when the nonresponsive reagents (Actin and Thromboplastin C+) were used in the assays. Specimens collected from all four treatment groups (no anticoagulant, IV heparin, oral anticoagulants, or both anticoagulant therapies) into 3.8% citrate had significantly higher PT values than those collected into 3.2% citrate with Innovin. When Thromboplastin C+ was used, only the IV heparin therapy treatment group had longer PT when collected in 3.8% rather than 3.2% citrate. Specimens collected from all four treatment groups into 3.8% citrate had significantly higher aPTT values than those collected into 3.2% citrate with Actin FS. When Actin was used, this was only true for patients on IV heparin therapy, with or without additional oral anticoagulants.

   Biospecimens

   • Bodily Fluid - Plasma
   • Bodily Fluid - Blood

   Preservative Types

   • None (Fresh)

   Diagnoses:

   • Normal
   • Not specified

   Platform:

   Analyte	Technology Platform
   Morphology	Hematology/ auto analyzer

   Pre-analytical Factors:

   Classification	Pre-analytical Factor	Value(s)
   Hematology/ auto analyzer Specific	Reaction solution	Innovin Thromboplastin C+ Actin FS Actin
   Biospecimen Acquisition	Anticoagulant	Sodium citrate Multiple concentrations evaluated
   Preaquisition	Other drugs	IV heparin IV heparin and oral anticoagulants Oral anticoagulants No anticoagulants

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