Increased incidence of positive tests for estrogen binding in mammary carcinoma specimens transported in liquid nitrogen.
Author(s): Muschenheim F, Furst JL, Bates HA
Publication: Am J Clin Pathol, 1978, Vol. 70, Page 780-2
PubMed ID: 717283 PubMed Review Paper? No
Purpose of Paper
Conclusion of Paper
Studies
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Study Purpose
The purpose of this study was to evaluate potential differences in estrogen binding activity among breast cancer specimens snap frozen in liquid nitrogen and transported either in liquid nitrogen or on dry ice to a laboratory for analysis. Specimens were not case-matched and included 43 cases shipped in liquid nitrogen and 218 shipped on dry ice. Information on the duration and type of transportation was not provided. Estradiol binding activity was quantified with a modified dextran charcoal method.
Summary of Findings:
ER binding, represented by a higher incidence of ER positive breast cancer cases, was significantly greater when snap frozen specimens were shipped in liquid nitrogen (69.3%) compared to those shipped on dry ice (56%; p<0.1). The threshold for a positive result was a cytosol content of greater than 3 fmol/mg estradiol-binding activity.
Biospecimens
Preservative Types
- Frozen
Diagnoses:
- Neoplastic - Carcinoma
Platform:
Analyte Technology Platform Protein Receptor binding Pre-analytical Factors:
Classification Pre-analytical Factor Value(s) Storage Specimen transport duration/condition Liquid nitrogen dry shipper
Dry ice