Immunologic studies of blood samples transported by a pneumatic tube system.

Author(s): Greendyke RM, Banzhaf JC, Pelysko S, Bauman B

Publication: Am J Clin Pathol, 1977, Vol. 68, Page 508-10

PubMed ID: 906982 PubMed Review Paper? No

Purpose of Paper

Conclusion of Paper

Studies

1. Study Purpose

   The purpose of this study was to determine the effects of pneumatic transport and vortexing on blood group antibody activity. The authors state that no effort was made to completely fill the tubes prior to pneumatic transport.

   Summary of Findings:

   Compared to hand delivered specimens, 42 of 79 clotted blood specimens showed reduced antibody activity and 75% showed hemolysis after pneumatic tube transport. The percentage of specimens showing reduced antibody activity did not depend on serologic reactivity. 34% of specimens decreases in antisera scores of 3 points or less and 19% showed a decrease of more than 3 points after pneumatic transport. The authors report no correlation between initial score and loss of potency due to pneumatic transport. There was no difference in reaction strengths after pneumatic transport in erythrocytes from 10 clotted blood specimens or 15 specimens anticoagulated with EDTA compared to hand delivered specimens. When serum was vortexed for as little as 30 sec, small decreases in antisera scores were observed for anti-A, anti-Le, anti-K antibodies, and after 4 min, anti-N also had a decreased score, but anti-Rh0-(D) scores were not affected.

   Biospecimens

   • Bodily Fluid - Blood

   Preservative Types

   • None (Fresh)

   Diagnoses:

   • Not specified

   Platform:

   Analyte	Technology Platform
   Protein	Hematology/ auto analyzer
   Protein	Immunoassay

   Pre-analytical Factors:

   Classification	Pre-analytical Factor	Value(s)
   Biospecimen Aliquots and Components	Biospecimen mixing	Not vortexed Vortexed 0.5 min Vortexed 1 min Vortexed 2 min Vortexed 4 min
   Storage	Within hospital transportation method	Hand-delivered Pneumatic tube system

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