The effects of 0.1 and 1.0 per cent erythrocytes and hemolysis on serum chemistry values.
Author(s): Laessig RH, Hassemer DJ, Paskey TA, Schwartz TH
Publication: Am J Clin Pathol, 1976, Vol. 66, Page 639-44
PubMed ID: 970364 PubMed Review Paper? No
Purpose of Paper
Conclusion of Paper
Studies
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Study Purpose
The purpose of this study was to determine the effects of contamination of serum with intact or hemolyzed red blood cells on serum chemistry values. Whole blood, with or without sodium saponin-induced hemolysis, was added to serum from healthy individuals and 40 clinical chemistry tests were performed.
Summary of Findings:
Contamination of serum with 0.1% or 1% non-hemolyzed erythrocytes had no effect on any of the 40 tests performed in this study. Contamination of serum with 1% hemolyzed erythrocytes turned the serum a cherry red color which would most likely lead to rejection of the specimen for analysis. Indeed this level of contamination led to gross changes in clinical chemistry values including increased calcium, phosphorus, total protein, magnesium, albumin, and direct cholesterol levels in addition to increases seen with 0.1% hemolyzed erythrocytes. Importantly, contamination of serum with 0.1% hemolyzed erythrocytes caused significant increases in lactate dehydrogenase, aspartate aminotransferase, creatine phosphokinase, alanine aminotransferase, iron, and iron-binding capacity, and potassium. Contamination at this level was not visually apparent and these specimens would most likely not be rejected for analysis in a clinical setting on the basis of hemolysis.
Biospecimens
Preservative Types
- None (Fresh)
Diagnoses:
- Normal
Platform:
Analyte Technology Platform Electrolyte/Metal Clinical chemistry/auto analyzer Electrolyte/Metal Flame emission photometry Carbohydrate Clinical chemistry/auto analyzer Steroid Clinical chemistry/auto analyzer Lipid Clinical chemistry/auto analyzer Protein Clinical chemistry/auto analyzer Protein Spectrophotometry Peptide Clinical chemistry/auto analyzer Small molecule Clinical chemistry/auto analyzer Pre-analytical Factors:
Classification Pre-analytical Factor Value(s) Biospecimen Aliquots and Components Biospecimen components Serum
0.1% erythrocytes in serum
0.1% hemolyzed erythrocytes in serum
1% erythrocytes in serum
1% hemolyzed erythrocytes in serum
Serum with sodium saponin
Biospecimen Aliquots and Components Hemolysis Hemolysate added
No hemolysate added
Chemically-induced