The influence of anticoagulation on acid-base status and blood-gas analysis.

Author(s): Börner U, Müller H, Höge R, Hempelmann G

Publication: Acta Anaesthesiol Scand, 1984, Vol. 28, Page 277-9

PubMed ID: 6430009 PubMed Review Paper? No

Purpose of Paper

Conclusion of Paper

Studies

1. Study Purpose

   The purpose of this study was to determine the effects of dilution and anticoagulant type on blood gas analysis. Artificial blood was created by mixing washed erythrocytes with serum. Aliquots of artificial tonometered blood were added to vials containing dried anticoagulant or saline.

   Summary of Findings:

   When any concentration of sodium heparin was added to tonometered artificial blood, pH and bicarbonate decreased (p<0.01), but the magnitude of the decrease was constant and did not depend on sodium heparin concentration (50-500 Units/mL). In contrast, addition of sodium citrate (2.5-25 mg/mL) or sodium EDTA (0.5-5.0 mg/mL) to tonometered artificial blood resulted in a linear decrease in pH and bicarbonate, which was significant (p<0.01) at concentrations greater than 5 and 0.5 mg/mL, respectively. Addition of sodium oxalate (0.8-4.0 mg/mL) led to small decreases in pH and bicarbonate at concentrations greater than 3.2 mg/mL, and sodium fluoride (1.25-5.0 mg/mL) led to small increases in pH and bicarbonate at concentrations greater than 2.5 mg/mL. However, the authors state that at usual concentrations, sodium fluoride and sodium oxalate had no effects on pH and bicarbonate, and the effects of heparin and sodium citrate were similar to one another. Dilution of the blood with as little as less than 0.5% saline led to significant decreases in pCO2 and bicarbonate. There were significant increases in PO2 and decreases in pH when blood was diluted by 12.5% and 50% or more, respectively.

   Biospecimens

   • Bodily Fluid - Blood

   Preservative Types

   • None (Fresh)

   Diagnoses:

   • Not specified

   Platform:

   Analyte	Technology Platform
   Gas	Clinical chemistry/auto analyzer
   Small molecule	Clinical chemistry/auto analyzer
   Small molecule	pH

   Pre-analytical Factors:

   Classification	Pre-analytical Factor	Value(s)
   Biospecimen Acquisition	Anticoagulant	Sodium citrate Sodium fluoride Sodium heparin Sodium oxalate Sodium EDTA
   Biospecimen Aliquots and Components	Biospecimen components	<5% saline 12.5% saline 25% saline 37.5% saline 50% saline 62.5% saline 75% saline 87.5% saline 100% saline

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