Welcome to the newest version of the Biospecimen Research Database (BRD), which accommodates Standard Operating Procedures (SOP). We encourage your contributions to our new SOP library!
The BRD is a free and publicly accessible database that contains peer-reviewed primary and review articles as well as SOPs in the field of human Biospecimen Science. Each literature curation has been created by a Ph.D.-level scientist to capture the following: (1) relevant parameters that include the biospecimen investigated (type and location, patient diagnosis), preservation method, analyte(s) of interest and technology platform(s) used for analysis; (2) the pre-analytical factors investigated, including those relating to pre-acquisition, acquisition, preservation, processing, storage, and analysis; and (3) an original summary of relevant results. Browse literature curations or submit specific queries using the Advanced Search page with keyword search for specific biomakers or genes, PubMed ID, or pre-analytical factor values (anticoagulant, fixative, reagent, etc).
SOPs are organized in a hierarchy system consisting of two tiers: (1) SOPs, established protocols; and (2) Biospecimen Evidence-based Practices (BEBP), procedural guidelines developed using literature evidence. SOP-tiered documents are a product of the Source organization specified. SOPs shared by external organizations are done so only with their consent, and have not been vetted by BBRB. SOP documents are searchable by keyword, or by curated fields (source organization, tier, applicable biospecimens, and topic) on the Search SOPs page. Related SOP documents are assembled in Compendiums, which are viewable on the SOP Compendiums page. You can also create your own Compendium and download SOPs together rather than individually.
We encourage you to submit SOPs from your lab or institution for inclusion in the BRD by clicking on the Submit an SOP tab or at email@example.com. Individuals and organizations that suggest articles for inclusion in the BRD will receive acknowledgement on the paper's curation page. Articles may be submitted by clicking on the Suggest a New Paper tab or via the email above. Feedback is also welcome.
The BRD is an initiative of the NCI Biorepositories and Biospecimen Research Branch (BBRB).
This study investigated the effect of underfilling lithium heparin and sodium heparin blood collection tubes on the measurement of ionized calcium in blood. Ionized calcium levels in blood were compared to those in serum from properly filled specimens collected in serum separator tubes (SSTs). Whole blood was collected from 20 healthy volunteers using a 21-gauge butterfly needle into blood collection tubes at different volumes. First, a “full-draw” of blood was collected in a 5 mL serum separator tube (SST) as a control and then 4.0, 2.0, 1.0, and 0.5 mL was collected into separate 4 mL Vacutainer sodium heparin (Na-Hep) blood tubes and 2.0, 1.0, and 0.5 of blood was collected in separate 2 mL Vacutainer lithium heparin (Li-Hep) tubes. All tubes were thoroughly mixed to distribute the heparin and minimize the formation of microclots. The serum was allowed to clot in the SST tubes for 60 min before centrifugation at 4000 rpm for 5 min. Ionized calcium concentrations and pH were measured using a blood gas analyzer within 15 min of collection for the Na-Hep and Li-Hep tubes and 70 min for the SST tubes.
|Technology Platform||Analyte||Clinical chemistry/auto analyzer||Small molecule|
A new Expert-Vetted Biospecimen Evidence-Based Practices document on blood collection and processing for cfDNA analysis is now available.
The top 5 most downloaded SOPs in the BRD in 2019 were supplied by:
GAPPS Repository: https://brd.nci.nih.gov/brd/sop/show/890More...