Welcome to the newest version of the Biospecimen Research Database (BRD), which accommodates Standard Operating Procedures (SOP). We encourage your contributions to our new SOP library!
The BRD is a free and publicly accessible database that contains peer-reviewed primary and review articles as well as SOPs in the field of human Biospecimen Science.
Each literature curation has been created by a Ph.D.-level scientist to capture the following: (1) relevant parameters that include the biospecimen investigated (type and location, patient diagnosis), preservation method, analyte(s) of interest and technology platform(s) used for analysis; (2) the pre-analytical factors investigated, including those relating to pre-acquisition, acquisition, preservation, processing, storage, and analysis; and (3) an original summary of relevant results. Browse literature curations or submit specific queries using the Advanced Search page with keyword search for specific biomakers or genes, PubMed ID, or pre-analytical factor values (anticoagulant, fixative, reagent, etc).
SOPs are organized in a hierarchy system consisting of two tiers: (1) SOPs, established protocols; and (2) Biospecimen Evidence-based Practices (BEBP), procedural guidelines developed using literature evidence. SOP-tiered documents are a product of the Source organization specified. SOPs shared by external organizations are done so only with their consent, and have not been vetted by BBRB. SOP documents are searchable by keyword, or by curated fields (source organization, tier, applicable biospecimens, and topic) on the Search SOPs page. Related SOP documents are assembled in Compendiums, which are viewable on the SOP Compendiums page. You can also create your own Compendium and download SOPs together rather than individually.
We encourage you to submit SOPs from your lab or institution for inclusion in the BRD by clicking on the Submit an SOP tab or at firstname.lastname@example.org. Individuals and organizations that suggest articles for inclusion in the BRD will receive acknowledgement on the paper's curation page. Articles may be submitted by clicking on the Suggest a New Paper tab or via the email above. Feedback is also welcome.
The BRD is an initiative of the NCI Biorepositories and Biospecimen Research Branch (BBRB).
The purpose of this study was to determine the effects of cold ischemia time on the antigenicity of 11 phosphoproteins in FFPE breast cancer tissue. A tissue microarray containing 93 breast cancer specimens (in duplicate) with a range of known cold ischemia times (25-415 min) was evaluated and quantitative immunofluorescence was performed using the Automated Quantitative Analysis (AQUA) method. Although initially included in the analysis, the authors report that statistical evaluation of phospho- human epidermal growth factor receptor 2 (Her2) staining was unable to be performed due to low numbers of Her2 positive cases and few specimens showing staining above the threshold to noise signal.
Visit BBRB's poster on the BRD at the ISBER Regional Meeting in Bethesda, MD on Nov 7th and 8th. http://meetings.isber.org/regional2016/
SOPs from NIH's Epidemiology & Genomics Research Program were added to the BRD. View them at http://brd.nci.nih.gov/brd/sop-compendium/show/501More...