The BRD is a free and publicly accessible database that contains peer-reviewed primary and review articles as well as SOPs in the field of human Biospecimen Science.
Each literature curation has been created by a Ph.D.-level scientist to capture the following: (1) relevant parameters that include the biospecimen investigated (type and location, patient diagnosis), preservation method, analyte(s) of interest and technology platform(s) used for analysis; (2) the pre-analytical factors investigated, including those relating to pre-acquisition, acquisition, preservation, processing, storage, and analysis; and (3) an original summary of relevant results. Browse literature curations or submit specific queries using the Advanced Search page with keyword search for specific biomakers or genes, PubMed ID, or pre-analytical factor values (anticoagulant, fixative, reagent, etc).
SOPs are organized in a hierarchy system consisting of two tiers: (1) SOPs, established protocols; and (2) Biospecimen Evidence-based Practices (BEBP), procedural guidelines developed using literature evidence. SOP-tiered documents are a product of the Source organization specified. SOPs shared by external organizations are done so only with their consent, and have not been vetted by BBRB. SOP documents are searchable by keyword, or by curated fields (source organization, tier, applicable biospecimens, and topic) on the Search SOPs page. Related SOP documents are assembled in Compendiums, which are viewable on the SOP Compendiums page. You can also create your own Compendium and download SOPs together rather than individually.
We encourage you to submit SOPs from your lab or institution for inclusion in the BRD by clicking on the Submit an SOP tab or at firstname.lastname@example.org. Individuals and organizations that suggest articles for inclusion in the BRD will receive acknowledgement on the paper's curation page. Articles may be submitted by clicking on the Suggest a New Paper tab or via the email above. Feedback is also welcome.
The BRD is an initiative of the NCI Biorepositories and Biospecimen Research Branch (BBRB).
This study compared the proteomic profile of PBMCs processed immediately and those obtained after a 24 h room temperature processing delay. Blood was collected from four healthy males into EDTA Vacutainer tubes and immediately separated into two aliquots. PBMCs were isolated by Ficoll-Paque density gradient centrifugation immediately or after 24 h at room temperature (22°C). Proteins were extracted from PBMC using RapiGest surfactant in triethylammonium bicarbonate and centrifuged at 20,000 × g for 20 min at 4°C. Total proteins were quantified using Qubit, reduced, trypsin digested, and labeled with Isobaric Tags for Relative and Absolute Quantitation (iTRAQ 8plex). Purified labeled peptides were quantified by hydrophilic interaction chromatography MS/MS.
|Technology Platform||Analyte||LC-MS or LC-MS/MS||Peptide||LC-MS or LC-MS/MS||Protein|
Click the links below to view the new SOPs that have recently been added to the BRD.
The May Editor's Choice Article for Journal of Histochemistry and Cytochemistry is "Impact of Preanalytical Factors on the Measurement of Tumor Tissue Biomarkers Using Immunohistochemistry" by Bagchi et al.