Welcome to the newest version of the Biospecimen Research Database (BRD), which accommodates Standard Operating Procedures (SOP). We encourage your contributions to our new SOP library!
The BRD is a free and publicly accessible database that contains peer-reviewed primary and review articles as well as SOPs in the field of human Biospecimen Science.
Each literature curation has been created by a Ph.D.-level scientist to capture the following: (1) relevant parameters that include the biospecimen investigated (type and location, patient diagnosis), preservation method, analyte(s) of interest and technology platform(s) used for analysis; (2) the pre-analytical factors investigated, including those relating to pre-acquisition, acquisition, preservation, processing, storage, and analysis; and (3) an original summary of relevant results. Browse literature curations or submit specific queries using the Advanced Search page with keyword search for specific biomakers or genes, PubMed ID, or pre-analytical factor values (anticoagulant, fixative, reagent, etc).
SOPs are organized in a hierarchy system consisting of two tiers: (1) SOPs, established protocols; and (2) Biospecimen Evidence-based Practices (BEBP), procedural guidelines developed using literature evidence. SOP documents are searchable by keyword, or by curated fields (source organization, tier, applicable biospecimens, and topic) on the Search SOPs page. Related SOP documents are assembled in Compendiums, which are viewable on the SOP Compendiums page. You can also create your own Compendium and download SOPs together rather than individually.
We encourage you to submit SOPs from your lab or institution for inclusion in the BRD by clicking on the Submit an SOP tab or at firstname.lastname@example.org. Individuals and organizations that suggest articles for inclusion in the BRD will receive acknowledgement on the paper's curation page. Articles may be submitted by clicking on the Suggest a New Paper tab or via the email above. Feedback is also welcome.
The BRD is an initiative of the NCI Biorepositories and Biospecimen Research Branch (BBRB).
The purpose of this study was to determine the effect of transporting blood in lithium heparin Vacutainer tubes by PTS on levels of LD in plasma and to investigate if the effects could be mitigated by wrapping the tube in bubble wrap or by collecting the blood by aspiration into a Monovette tube. Paired lithium heparin blood specimens from 79 patients in the nursery unit were sent to the laboratory by PTS or were hand-delivered. From 31 patients, an additional specimen was collected into a Vacutainer and bubble wrapped prior to transport, and from 28 patients, an additional specimen was collected by aspiration into a lithium heparin Monovette tube. Specimens sent by PTS were inserted into a plastic bag inside a plastic parachute and sent along a 1100 m path at a maximum of 6 m/s. They arrived at the laboratory after cessation of propulsion onto a cushioned pad and were centrifuged with the hand-delivered specimen. Transport time by either method was <10 min.
|Technology Platform||Analyte||Clinical chemistry/auto analyzer||Protein|
Look for a link to the BRD on the bottom of the PubMed abstract page, under "LinkOut-more resources".
The review appears in Cancer Research's OnlineFirst section, and both summarizes the challenges associated with molecular analysis of FFPE biospecimens as well as highlights analytical techniques and parameters that result in strong concordance with a fresh or frozen cohort.More...