Welcome to the newest version of the Biospecimen Research Database (BRD), which accommodates Standard Operating Procedures (SOP). We encourage your contributions to our new SOP library!
The BRD is a free and publicly accessible database that contains peer-reviewed primary and review articles as well as SOPs in the field of human Biospecimen Science.
Each literature curation has been created by a Ph.D.-level scientist to capture the following: (1) relevant parameters that include the biospecimen investigated (type and location, patient diagnosis), preservation method, analyte(s) of interest and technology platform(s) used for analysis; (2) the pre-analytical factors investigated, including those relating to pre-acquisition, acquisition, preservation, processing, storage, and analysis; and (3) an original summary of relevant results. Browse literature curations or submit specific queries using the Advanced Search page with keyword search for specific biomakers or genes, PubMed ID, or pre-analytical factor values (anticoagulant, fixative, reagent, etc).
SOPs are organized in a hierarchy system consisting of two tiers: (1) SOPs, established protocols; and (2) Biospecimen Evidence-based Practices (BEBP), procedural guidelines developed using literature evidence. SOP-tiered documents are a product of the Source organization specified. SOPs shared by external organizations are done so only with their consent, and have not been vetted by BBRB. SOP documents are searchable by keyword, or by curated fields (source organization, tier, applicable biospecimens, and topic) on the Search SOPs page. Related SOP documents are assembled in Compendiums, which are viewable on the SOP Compendiums page. You can also create your own Compendium and download SOPs together rather than individually.
We encourage you to submit SOPs from your lab or institution for inclusion in the BRD by clicking on the Submit an SOP tab or at firstname.lastname@example.org. Individuals and organizations that suggest articles for inclusion in the BRD will receive acknowledgement on the paper's curation page. Articles may be submitted by clicking on the Suggest a New Paper tab or via the email above. Feedback is also welcome.
The BRD is an initiative of the NCI Biorepositories and Biospecimen Research Branch (BBRB).
This study examined whether the duration of FFPE block storage affects successful detection of HER-2 genomic amplification by DISH. Ovarian specimens were surgically obtained from 92 patients diagnosed with epithelial ovarian cancer and fixed in neutral buffered formalin for unspecified durations. FFPE specimens stored for 10 y or less were stored at the same site as collection, while those stored for longer than 10 y were stored offsite. FFPE sections (3 µm thick) were used for DISH staining with a commercial probe cocktail and an automated stainer. When necessary, the efficiency of different optimization techniques was evaluated using archival FFPE specimens. These optimization steps focused on durations of cell conditioning, chromagen incubation, counterstain incubation, as well as the use and duration of protease 2 or 3 incubations, and the temperature of the stringency wash.
|Technology Platform||Analyte||In situ hybridization||DNA|
Stop by and visit BBRB's poster on the BRD and the Biospecimen Evidence-Based Practices document series at the ISBER 2017 Annual Meeting on May 11th.
The top 5 most downloaded SOPs in the BRD in 2016 were supplied by
GAPPS Repository: https://brd.nci.nih.gov/brd/sop/show/890
UCHC Tissue Repository: https://brd.nci.nih.gov/brd/sop/show/841More...