The BRD is a free and publicly accessible database that contains peer-reviewed primary and review articles as well as SOPs in the field of human Biospecimen Science.
Each literature curation has been created by a Ph.D.-level scientist to capture the following: (1) relevant parameters that include the biospecimen investigated (type and location, patient diagnosis), preservation method, analyte(s) of interest and technology platform(s) used for analysis; (2) the pre-analytical factors investigated, including those relating to pre-acquisition, acquisition, preservation, processing, storage, and analysis; and (3) an original summary of relevant results. Browse literature curations or submit specific queries using the Advanced Search page with keyword search for specific biomakers or genes, PubMed ID, or pre-analytical factor values (anticoagulant, fixative, reagent, etc).
SOPs are organized in a hierarchy system consisting of two tiers: (1) SOPs, established protocols; and (2) Biospecimen Evidence-based Practices (BEBP), procedural guidelines developed using literature evidence. SOP-tiered documents are a product of the Source organization specified. SOPs shared by external organizations are done so only with their consent, and have not been vetted by BBRB. SOP documents are searchable by keyword, or by curated fields (source organization, tier, applicable biospecimens, and topic) on the Search SOPs page. Related SOP documents are assembled in Compendiums, which are viewable on the SOP Compendiums page. You can also create your own Compendium and download SOPs together rather than individually.
We encourage you to submit SOPs from your lab or institution for inclusion in the BRD by clicking on the Submit an SOP tab or at firstname.lastname@example.org. Individuals and organizations that suggest articles for inclusion in the BRD will receive acknowledgement on the paper's curation page. Articles may be submitted by clicking on the Suggest a New Paper tab or via the email above. Feedback is also welcome.
The BRD is an initiative of the NCI Biorepositories and Biospecimen Research Branch (BBRB).
This study compared PT; DRVTT; and APTT; von Willebrand factor activity; and levels of Protein C, Protein S, antithrombin, and factors FII, FV, FVII, FVIII, FIX, FX, FXI, and FXII in plasma stored frozen as 0.4, 0.8 and 1.6 mL aliquots in 2 mL tubes. The authors also compared results in plasma stored as 20% filled 2 and 5 mL tubes (0.4 mL and 1 mL, respectively). Blood from 85 patients (diagnosis was not specified, but 7 were receiving unfractionated heparin and 13 vitamin K antagonists) was collected via venipuncture into tubes containing 0.109 M citrate. Platelet poor plasma was separated by centrifugation twice at 2500 g for 15 minutes and stored as 0.4, 0.8 and 1.6 mL aliquots in 2 mL microtubes and as 1 mL aliquots in 5 mL tubes for 3 months at -80°C. Plasma was thawed in a 37°C water bath for 5 min before coagulation testing on a STAR Max coagulometer and AcuStar instrument (von Willebrand factor activity only).
|Technology Platform||Analyte||Hematology/ auto analyzer||Morphology||Hematology/ auto analyzer||Protein||Hematology/ auto analyzer||Glycoprotein|
The expert-vetted NCI BEBP details blood collection and processing procedures specifically tailored for miRNA analysis. View the BEBP on BBRB’s website and in the BRD!
Announcement of a new NCI Biospecimen Evidence-Based Practices on blood collection and processing for miRNA analysis is available ahead of print in Clinical Chemistry Laboratory Medicine.More...