Welcome to a newly released version of the Biospecimen Research Database (BRD)! This version marks the first release of Standard Operating Procedure (SOP) functionality and content. We encourage your contributions to this new SOP library.
The BRD is a free and publicly accessible database that contains peer-reviewed primary and review articles as well as SOPs in the field of human Biospecimen Science.
Each literature curation has been created by a Ph.D.-level scientist to capture the following: (1) relevant parameters that include the biospecimen investigated (type and location, patient diagnosis), preservation method, analyte(s) of interest and technology platform(s) used for analysis; (2) the pre-analytical factors investigated, including those relating to pre-acquisition, acquisition, preservation, processing, storage, and analysis; and (3) an original summary of relevant results. Browse literature curations or submit specific queries using the Advanced Search page with keyword search for specific biomakers or genes, PubMed ID, of pre-analytical factor values (anticoagulant, fixative, reagent, etc).
SOPs are organized in a hierarchy system consisting of three tiers: (1) SOPs, established protocols; (2) Biospecimen Evidence-based Practices (BEBP), procedural guidelines developed using literature evidence; and (3) Expert-vetted, evidence-based guidelines vetted by a panel of experts in the field. SOP documents are searchable by keyword, or by curated fields (source organization, tier, applicable biospecimens, and topic) on the Search SOPs page. Related SOP documents are assembled in Compendiums, which are viewable on the SOP Compendiums page.
We encourage you to submit SOPs from your lab or institution for inclusion in the BRD at email@example.com. Feedback is also welcome. Article suggestions for inclusion in BRD may be submitted by clicking on the Suggest a New Paper tab or via the email above.
The BRD is an initiative of the NCI Biorepositories and Biospecimen Research Branch (BBRB).
The purpose of this study was to determine the effects of anticoagulant use and type, miRNA extraction and purification method, and analytical variables including template amount and polymerase type on the quantitation of miR-16 and miR-223 in plasma and serum. Diluted plasma and serum specimens were analyzed immediately or were flash-frozen and stored at -80 degrees C. After RNA extraction, samples were used immediately or stored at -70 degrees C. The stability of miR-16 (isolated from cultured BC3 cells) was evaluated during incubation of whole blood and plasma at 10 degrees C.
|Technology Platform||Analyte||Real-time qRT-PCR||RNA||RT-PCR||RNA|
A new review summarizing the effects of FFPE processing factors on nucleic acid, protein, and morphological endpoints appears in the November 2014 issue of Arch Pathol Lab Med. The review was drafted using references housed in the BRD.
The ISBER Biospecimen Science Working Group has compiled hundreds of biospecimen science references from both the clinical and the environmental sectors: click here!More...