Validation of tissue microarray technology in squamous cell carcinoma of the esophagus.

Author(s): Boone J, van Hillegersberg R, van Diest PJ, Offerhaus GJ, Rinkes IH, Kate FJ

Publication: Virchows Arch, 2008, Vol. 452, Page 507-14

PubMed ID: 18392848 PubMed Review Paper? No

Purpose of Paper

Conclusion of Paper

Studies

1. Study Purpose

   The purpose of this study was to determine the effects of analyzing TMAs rather than entire sections on grade of tumor differentiation and IHC staining in ESCC. The array contained 3 cores from each tumor. Any specimens for which less than 2 cores stained or in which the cores yielded discrepant results were excluded.

   Summary of Findings:

   The grade of tumor differentiation determined by H&E staining of tissue microarrays and whole sections was concordant in 78% of cases. IHC staining for CK5/6, CK14, e-cadherin, ki-67 and p53 was concordant between the TMA and entire sections in 98%, 85%, 72%, 79%, and 87% of cases, respectively. Generally, staining of individual cores from the same tumor was concordant, but in 5% of specimens the scores for CK14 differed between cores.

   Biospecimens

   • Tissue - Esophagus

   Preservative Types

   • Formalin

   Diagnoses:

   • Neoplastic - Carcinoma

   Platform:

   Analyte	Technology Platform
   Morphology	H-and-E microscopy
   Protein	Immunohistochemistry
   Protein	Tissue microarray

   Pre-analytical Factors:

   Classification	Pre-analytical Factor	Value(s)
   Immunohistochemistry Specific	Targeted peptide/protein	CK5/6 CK14 E-Cadherin Ki-67 (MIB1) p53

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