Cancer Diagnosis Program | Biorepositories and Biospecimen Research Branch

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Dehydration of blood platelets by zeodration: in vitro characterization and hemostatic properties in vivo.

Author(s): Donnet T, Ravanat C, Eckly A, Maurer E, Alame G, Ziessel C, Mangin PH, Freund M, Cazenave JP, Gachet C, Rendu F

Publication: Transfusion, 2015, Vol. 55(9), Page 2207-18

PubMed ID: 25856501 PubMed Review Paper? No

Purpose of Paper

Conclusion of Paper

Studies

1. Study Purpose

   The purpose of this study was to compare morphology, Plt activation, aggregation and clotting properties of fresh and rehydrated zPlts. Plts were isolated from whole blood from 4-10 donors and were dehydrated at room temperature by Zeodration and stored at room temperature for an unspecified duration. Specimens were rehydrated at RT by controlled humidity increases from 50% to 99% over 30 minutes and then resuspended in distilled water.

   Summary of Findings:

   After rehydration, 85% of plts in the fresh specimen were recovered from the zeodrated specimen. The rehydrated zPlts had similar distribution profile to fresh plts, but electron microscopy showed them to no longer be discoid and to have blebs in the cellular membranes. Importantly, while 1% of fresh Plts had permeable membranes, 96% of rehydrated zPlts had permeable membranes. Rehydrated zPlts had lower expression of GPIb, and higher binding of annexin V and PAC1, and higher expression of CD63, fibrinogen and VWF than fresh Plts (p<0.05, all), but comparable expression of GPIIbIIIa, GPX, and GPVI.  While rehydrated zPlts had non-significantly less P-selectin and CD40L surface expression than fresh Plts, the supernatants of zPlts contained significantly more soluble CD40L and P-selectin. Rehydrated zPlts did not spontaneously agglutinate or aggregate, and in the presence of ristocetin, comparable agglutination was observed for fresh and zPlts. However, zPlts did not aggregate in response to ADP, and collagen and thrombin induced zPlt aggregation were slower than that observed for fresh Plts. zPlts did not attach as well as fresh Plts to a VWF coated chamber, but zPlts were still able to form a thrombus comparable to that obtained with fresh Plts, and in the presence of calcium, with or without tissue factor, thrombin generation of zPlts occurred faster.

   Biospecimens

   • Bodily Fluid - Blood

   Preservative Types

   • None (Fresh)
   • Other Preservative

   Diagnoses:

   • Not specified

   Platform:

   Analyte	Technology Platform
   Cell count/volume	Flow cytometry
   Protein	ELISA
   Morphology	Flow cytometry
   Glycoprotein	Flow cytometry
   Morphology	Electron microscopy
   Protein	Flow cytometry

   Pre-analytical Factors:

   Classification	Pre-analytical Factor	Value(s)
   Biospecimen Preservation	Type of fixation/preservation	Zeodration None (fresh)

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