In vitro function of platelets treated with ultraviolet C light for pathogen inactivation: a comparative study with nonirradiated and gamma-irradiated platelets.
Author(s): Tynngård N, Trinks M, Berlin G
Publication: Transfusion, 2015, Vol. 55(6), Page 1169-77
PubMed ID: 25524519 PubMed Review Paper? No
Purpose of Paper
The purpose of this paper was to determine the effects of gamma (γ)-irradiation or ultraviolet C (UVC) illumination and room temperature storage on platelet (plt) function.
Conclusion of Paper
Mean plt volume (MPV), pH, glucose levels, hypotonic shock response (HSR), and maximum clot elasticity (G'Max) were lower and mean G'Max change was smaller in UVC-treated specimens than untreated or γ-irradiated specimens. On the other hand, lactate levels and lactate dehydrogenase (LDH) levels were higher and time to G'Max was longer in UVC-treated specimens than untreated or γ-irradiated specimens. The partial pressure oxygen (pO2) was lower in UVC-treated than in γ-irradiated specimens. Regardless of treatment, room temperature storage of plts reduced the partial pressure carbon dioxide (pCO2), glucose and glycoprotein IIb (GPIIb) levels and increased lactate levels, G'Max and time to G'Max. Storage of plts also decreased pH and increased LDH in most specimens, but the effect was not significant in UVC-illuminated specimens stored for 7 days. Generally, pO2, plt concentration, P-selectin expression, and HSR were unaffected by storage of plts, but some effects were noted in UVC-treated specimens. Swirling and clotting time were unaffected by treatment and storage duration.
Studies
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Study Purpose
The purpose of this study was to determine the effects of γ-irradiation or UVC illumination on plt function after room temperature storage. Buffy coats were obtained from the whole blood of 50 healthy donors. Buffy coats were held overnight at room temperature, and pools of five specimens each were processed into a total of 10 plt concentrates (PC) in SSP+ using the Terumo OrbiSac system. Three PCs were pooled and then divided to make the 3 treatment groups. On day 1, specimens were subjected to 30 sec of UVC illumination (0.2 J/cm2) in a different container, gamma-irradiated with 25 Gy in the original container, or left untreated. PCs were stored at 22±2°C in polyvinyl chloride containers on an agitator.
Summary of Findings:
MPV, pH, glucose, HSR, and G'Max were lower and mean G'Max change was smaller, while lactate and LDH levels were higher and time to G'Max was longer in UVC-treated specimens than untreated or γ-irradiated specimens (p<0.05, all). The pO2 was lower in UVC-treated than in γ-irradiated specimens (p<0.05), but neither was significantly different than in untreated controls. Compared to day 1, PCs stored for 5 or 7 days at room temperature had lower pCO2 and glucose levels, higher lactate levels and G'Max, and longer time to G'Max, regardless of treatment (p<0.05, all durations for all treatments), and specimens stored for 7 days had reduced GPIIb expression (p<0.05, all). On day 5, specimens from each of the three treatment groups had lower pH and higher LDH levels than on day 1, but on day 7, only untreated and γ-irradiated specimens had significantly lower pH (p<0.05, both) and higher LDH levels (p<0.05, both) than on day 1. Generally, pO2, plt concentration, P-selectin expression, and HSR were unaffected by storage, but in UVC-treated specimens on day 5, pO2 was lower and HSR was higher than on day 1 (p<0.05, both), and on day 7, plt concentration was lower, and P-selectin expression and HSR were higher than on day 1 (p<0.05, all). Swirling and clotting time were unaffected by treatment and storage duration.
Biospecimens
Preservative Types
- None (Fresh)
Diagnoses:
- Normal
Platform:
Analyte Technology Platform Morphology Macroscopic observation Morphology Hematology/ auto analyzer Cell count/volume Hematology/ auto analyzer Protein Clinical chemistry/auto analyzer Gas Clinical chemistry/auto analyzer Small molecule Clinical chemistry/auto analyzer Carbohydrate Clinical chemistry/auto analyzer Cell count/volume Flow cytometry Pre-analytical Factors:
Classification Pre-analytical Factor Value(s) Storage Time at room temperature 1 day
5 days
7 days
Biospecimen Aliquots and Components Blood processing method Untreated
Gamma-irradiated
UVC-light treated