Accumulation of bioactive lipids during storage of blood products is not cell but plasma derived and temperature dependent.

Author(s): Vlaar AP, Kulik W, Nieuwland R, Peters CP, Tool AT, van Bruggen R, Juffermans NP, de Korte D

Publication: Transfusion, 2011, Vol. 51, Page 2358-66

PubMed ID: 21575006 PubMed Review Paper? No

Purpose of Paper

Conclusion of Paper

Studies

1. Study Purpose

   The purpose of this study was to determine the effects of storage solution and refrigerated storage on neutrophil priming activity and levels of lysoPCs and PCs in RBCs. Citrate-phosphate-dextrose (CPD) blood was stored at room temperature for 12-18 h before preparation of RBCs. RBC supernatants were frozen at -80 degrees C until analysis.

   Summary of Findings:

   LysoPC and PCs did not increase in supernatants during refrigerated storage of RBCs in SAGM. Further, the supernatant neutrophil-priming activity was unaffected by refrigerated storage of RBCs in SAGM. LysoPC concentrations were higher in the supernatants of RBCs in CPD plasma than RBCs in SAGM on day 0, but no further increases were observed in LysoPC levels in the supernatant or RBCs in plasma during refrigerated storage.

   Biospecimens

   • Cell - Red Blood Cells

   Preservative Types

   • Other Preservative

   Diagnoses:

   • Not specified

   Platform:

   Analyte	Technology Platform
   Lipid	HPLC-MS
   Small molecule	HPLC-MS
   Protein	Fluorometry

   Pre-analytical Factors:

   Classification	Pre-analytical Factor	Value(s)
   Storage	Storage duration	0 days 7 days 21 days 35 days 42 days 49 days
   Storage	Short-term storage solution	SAGM CPD plasma with adenine

   View more

2. Study Purpose

   The purpose of this study was to determine the effects of storage solution and room temperature storage on neutrophil priming activity and levels of lysoPCs and PCs in Plts. CPD blood was stored at room temperature for 12-18 h before preparation of Plts. Plts were stored at 22 degrees C with horizontal shaking. Plt supernatants were frozen at -80 degrees C until analysis.

   Summary of Findings:

   LysoPCs increased and PCs decreased with room temperature storage of Plts in plasma (p<0.01, all). Further, the neutrophil-priming activity of supernatants of Plts in plasma increased with increasing room temperature storage (p<0.05). Replacement of 65% of the plasma with SSP+ significantly decreased the LysoPC concentration and neutrophil-priming activity of Plt supernatants (p<0.05), but further storage of Plts in 65% SSP+ only led to a slight decrease in PCs (p<0.05) and no further changes in LysoPCs. When 95% of the plasma for Plt storage was replaced by SSP+, the LysoPC concentration was even lower than when only 65% of the plasma was replaced, and, similarly to when only 65% of the plasma was replaced, PCs in the Plt supernatant declined with room temperature storage (p<0.05). However, the supernatants of Plts stored in 95% SSP+ for 7 days had higher neutrophil-priming activity than Plts in 100% plasma or 65% SSP+.

   Biospecimens

   • Bodily Fluid - Blood

   Preservative Types

   • None (Fresh)

   Diagnoses:

   • Normal

   Platform:

   Analyte	Technology Platform
   Lipid	HPLC-MS
   Small molecule	HPLC-MS
   Protein	Fluorometry

   Pre-analytical Factors:

   Classification	Pre-analytical Factor	Value(s)
   Storage	Storage duration	0 days 5 days 7 days 9 days
   Storage	Short-term storage solution	100% plasma 65% SSP+ and 35% plasma 95% SSP+ and 5% plasma

   View more

3. Study Purpose

   The purpose of this study was to determine the effects of collection method, storage solution and room temperature storage on neutrophil priming activity and levels of lysoPCs and PCs in plasma. Cell depleted plasma, collected by apheresis, was compared to plasma obtained from CPD blood that was stored at room temperature for 12-18 h before plasma preparation. PCs were stored at 22 degrees C with horizontal shaking. PC supernatants were frozen at -80 degrees C until analysis.

   Summary of Findings:

   LysoPCs increased and PCs decreased in plasma by day 5 of room temperature storage (p<0.05, all), but there was no corresponding increase in neutrophil-priming activity, and there were no changes in LysoPCs or PCs during refrigerated storage of plasma. The authors report similar effects of room temperature storage on the LysoPC and PC content of cell-free plasma obtained by apheresis to those obtained using plasma from whole blood. Addition of soluble PLA2 inhibitors did not affect LysoPC accumulation during room temperature storage of plasma, and the authors report similar findings with the addition of cytosolic PLA2, but the data was not shown.

   Biospecimens

   • Bodily Fluid - Plasma
   • Bodily Fluid - Blood

   Preservative Types

   • None (Fresh)

   Diagnoses:

   • Normal

   Platform:

   Analyte	Technology Platform
   Protein	Fluorometry
   Lipid	HPLC-MS
   Small molecule	HPLC-MS

   Pre-analytical Factors:

   Classification	Pre-analytical Factor	Value(s)
   Biospecimen Acquisition	Method of fluid acquisition	Apheresis Venipuncture
   Storage	Short-term storage solution	Cytosolic PLA2 inhibitor added Soluble PLA2 inhibitor added No inhibitor added
   Storage	Storage duration	0 days 5 days 7 days 9 days
   Storage	Storage temperature	4 degrees C 22 degrees C
   Biospecimen Aliquots and Components	Centrifugation	Centrifugation delays investigated

   View more