Profiling of alterations in platelet proteins during storage of platelet concentrates.
Author(s): Thiele T, Steil L, Gebhard S, Scharf C, Hammer E, Brigulla M, Lubenow N, Clemetson KJ, Völker U, Greinacher A
Publication: Transfusion, 2007, Vol. 47, Page 1221-33
PubMed ID: 17581157 PubMed Review Paper? No
Suggested by: ISBER
Purpose of Paper
The purpose of this paper was to determine the effects of platelet collection method and storage at room temperature on the platelet proteome.
Conclusion of Paper
Pooled platelets obtained by aphresis had more changes in spot intensity after storage than those obtained from buffy coat. Within the first 9 days of storage at 22 degrees C, only 5 spots were significantly altered in both pooled specimen types, and the relative intensities of all spots remained within 10-fold of baseline. After 15 days of storage, 78 spots were significantly affected in both pooled specimens types, and 100-fold or greater changes in relative spot intensities were found. Mass spectrometry revealed that commonly used normalization proteins such as B-actin and fibrinogen were affected by storage as early as day 5.
Studies
-
Study Purpose
The purpose of this study was to determine the effects of platelet collection method and storage at room temperature on the platelet proteome.
Summary of Findings:
A pH range for specimen separation of 4-7 was found to give the best resolution and cover the majority of the platelet proteome. Within the first 9 days of storage at 22 degrees C, very few significant changes were identified and the relative intensities of all spots remained within 10-fold of baseline. After 15 days of storage, a large number of proteins were significantly affected and 100-fold or greater changes in relative spot intensities were found. By day 15, 296 spots were significantly different from baseline, 78 of which differed in both apheresis and buffy coat-derived pooled specimens. In general, the largest number of changes in relative intensities was found in one individual specimen. Pooled platelets obtained by apheresis were more affected by storage than those obtained from buffy coat. Mass Spectrometry revealed that commonly used normalization proteins such as B-actin and fibrinogen were affected by storage as early as day 5.
Biospecimens
Preservative Types
- None (Fresh)
Diagnoses:
- Not specified
Platform:
Analyte Technology Platform Peptide MALDI-TOF MS Peptide LC-MS or LC-MS/MS Protein LC-MS or LC-MS/MS Protein MALDI-TOF MS Protein Western blot Peptide 1D/2D gels Protein 1D/2D gels Pre-analytical Factors:
Classification Pre-analytical Factor Value(s) Storage Time at room temperature 0 days
1 day
2 days
3 days
5 days
9 days
15 days
Biospecimen Acquisition Method of fluid acquisition Apheresis
Venipuncture
1D/2D gels Specific pH 3-10
6-11
4-7
Biospecimen Aliquots and Components Blood processing method Apheresis
Buffy coat method