Comparison of soluble thrombomodulin, von Willebrand factor, tPA/PAI-1 complex, and high-sensitivity CRP concentrations in serum, EDTA plasma, citrated plasma, and acidified citrated plasma (Stabilyte) stored at -70 degrees C for 8-11 years.
Author(s): Nilsson TK, Boman K, Jansson JH, Thøgersen AM, Berggren M, Broberg A, Granlund A
Publication: Thromb Res, 2005, Vol. 116, Page 249-54
PubMed ID: 15935834 PubMed Review Paper? No
Purpose of Paper
Conclusion of Paper
Studies
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Study Purpose
The purpose of this study was to determine the effects of collection tube type on sTM, vWF, tPA/PAI-1 complex, and hsCRP concentration determination in serum and plasma. All specimens were stored for 8-11 years at -70 degrees C prior to analysis.
Summary of Findings:
When plotted against paired citrate specimens, sTM was very unstable in serum and marginally unstable in K3EDTA tubes, but showed increased stability in the more highly acidic Stabilyte tubes. vWF concentrations were similar across tube types, but serum specimens had not been diluted 1:10 indicating a substantial loss of vWF immunoreactivity in serum. While no change in the slopes of tPA/PAI-1 in citrate tubes versus any other tube were observed, the values were lower in specimens collected in Stabilyte tubes than those collected in other tube types. The authors report that results for hsCRP in serum and K3EDTA tubes did not differ from what was expected based on dilution effect. The authors conclude that Stabilyte tubes are the best choice for biobanks investigating fibrinolytic factors or other aspects of the clotting system.
Biospecimens
Preservative Types
- Frozen
Diagnoses:
- Cardiovascular Disease
Platform:
Analyte Technology Platform Protein ELISA Glycoprotein ELISA Protein Clinical chemistry/auto analyzer Pre-analytical Factors:
Classification Pre-analytical Factor Value(s) Biospecimen Aliquots and Components Blood and blood products Plasma
Serum
Storage Storage temperature -70 degrees C
Storage Storage duration 8-11 years
Biospecimen Acquisition Anticoagulant Sodium citrate
Potassium EDTA
Buffered acidic citrate