Cancer Diagnosis Program | Biorepositories and Biospecimen Research Branch

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Effects of sample storage time, temperature and syringe type on blood gas tensions in samples with high oxygen partial pressures.

Author(s): Pretto JJ, Rochford PD

Publication: Thorax, 1994, Vol. 49, Page 610-2

PubMed ID: 8016801 PubMed Review Paper? No

Purpose of Paper

Conclusion of Paper

Studies

1. Study Purpose

   The purpose of this study was to determine the effects of storage container and temperature on blood gas analysis. Heparinized blood was tonometered with 95% oxygen and 5% carbon dioxide to have a high PO2 before transfer into the storage containers, which were periodically agitated.

   Summary of Findings:

   The PCO2 increased, and the PO2 and pH decreased with storage, and the rate of change was greater when blood was stored at room temperature than in ice water. The PO2 decreased significantly in specimens stored at room temperature for 5 min, regardless of syringe type, but was generally the most stable in specimens stored in glass syringes and least stable in Preza-Pak syringes. The increases in PCO2 were first significant after at least 40 min of storage and were greatest when specimens were stored in glass or Preza-Pak II syringes. The PCO2 decreased slightly in the first 20 min in specimens stored in OS90 syringes, but then remained relatively stable.

   Biospecimens

   • Bodily Fluid - Blood

   Preservative Types

   • None (Fresh)

   Diagnoses:

   • Not specified

   Platform:

   Analyte	Technology Platform
   Gas	Radiometer

   Pre-analytical Factors:

   Classification	Pre-analytical Factor	Value(s)
   Storage	Storage duration	0 min 5 min 10 min 20 min 40 min 60 min 90 min 120 min
   Storage	Storage temperature	Room temperature (21-23 degrees C) Ice water
   Storage	Type of storage container	Glass syringe Plastic Marquest Quick syringe Plastic Terumo Preza-Pak II syringe Plastic Radiometer QS90 syringe

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