Cancer Diagnosis Program | Biorepositories and Biospecimen Research Branch

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The effect of storage conditions on salivary cortisol concentrations using an enzyme immunoassay.

Author(s): Nalla AA, Thomsen G, Knudsen GM, Frokjaer VG

Publication: Scand J Clin Lab Invest, 2015, Vol. 75, Page 92-5

PubMed ID: 25510953 PubMed Review Paper? No

Purpose of Paper

Conclusion of Paper

Studies

1. Study Purpose

   The purpose of this study was to determine the effects of storage temperature and freeze-thaw cycling on salivary cortisol levels. 3 saliva specimens were collected from 13 healthy volunteers using Salivette tubes and swabs within 5 min of each other either between 8-10 AM or 11 AM-Noon. Saliva was centrifuged and aliquoted into 6 tubes for storage at -80°C, -18°C, -4°C, 4°C, or room temperature for 72 h. The last tube was stored at -18°C for 72, stored in an envelope at room temperature for 72 h and then frozen at -80°C for an unspecified duration (2 freeze-thaw cycles).

   Summary of Findings:

   Storage temperature and freeze-thaw cycling did not significantly impact salivary cortisol levels. While seven (of the 78) specimens had a coefficient of variance (CV) >10% between replicates, exclusion of those 7 did not affect significance.

   Biospecimens

   • Bodily Fluid - Saliva

   Preservative Types

   • None (Fresh)
   • Frozen

   Diagnoses:

   • Normal

   Platform:

   Analyte	Technology Platform
   Steroid	Immunoassay

   Pre-analytical Factors:

   Classification	Pre-analytical Factor	Value(s)
   Storage	Storage temperature	-80°C -18°C -4°C 4°C Room temperature
   Storage	Freeze/thaw cycling	0 cycles 1 cycle 2 cycles

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