Six-day stability of erythrocyte and reticulocyte parameters in-vitro: A comparison of blood samples from healthy, iron-deficient, and thalassemic individuals.
Author(s): Sudmann-Day ÅA, Piehler A, Klingenberg O, Urdal P
Publication: Scand J Clin Lab Invest, 2015, Vol. 75, Page 247-53
PubMed ID: 25608597 PubMed Review Paper? No
Purpose of Paper
The purpose of this paper was to determine the effects of storage duration and temperature and diagnosis on hematological parameters in blood.
Conclusion of Paper
With room temperature storage, mean corpuscular volume (MCV), red cell distribution width (RDW) and reticulocyte counts increased, but the magnitude of the changes depended on diagnosis. During room temperature storage, hemoglobin (Hb), reticulocyte Hb equivalent (Ret-He) and red blood cell (RBC) counts increased in blood from iron-deficient and thalessemic individuals, but Hb remained stable and RBC counts and Ret-He declined slightly (p<0.0002) in blood from healthy individuals. The effect on Ret-He appeared to be dependent on initial levels, with increases noted when baseline was ≤24 pg and decreases noted when baseline was >24 pg. Room temperature storage of blood had no effects on mean corpuscular hemoglobin (MCH), regardless of diagnosis. Stability of all analytes was comparable to or improved when blood was refrigerated rather than stored at room temperature.
Studies
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Study Purpose
The purpose of this study was to determine the effects of room temperature storage and diagnosis on hematological parameters in EDTA blood from 8 healthy, 8 iron-deficient and 11 thalessemic patients.
Summary of Findings:
During room temperature storage, Hb and RBC counts increased in blood from iron-deficient and thalessemic individuals (p<0.0002, all), but Hb remained stable and RBC counts declined slightly (p<0.0002) in blood from healthy individuals. While MCV increased with storage in all groups (p<0.0002, all), the rate of increase was lower in specimens from iron-deficient patients than healthy individuals (p<0.008). RDW increased with room temperature storage for all groups (p<0.002, all), but the increase was greater in specimens from healthy individuals than those from iron deficient or thalessemic patients (p<0.008, both). The number of reticulocytes increased when specimens were stored at room temperature for 3 days or more (p<0.002, all), but the increase was higher in specimens from thalessemic patients than healthy individuals (p<0.008). Ret-He increased during room temperature storage of blood from thalessemic and iron-deficient individuals (p<0.002 and p=0.047, respectively) and decreased with storage of blood from healthy individuals (p<0.002), with significant differences between diagnosis (p<0.008). However the effect on Ret-He appeared to be dependent on initial levels, with increases noted when baseline was ≤24 pg and decreases noted when baseline was >24 pg (p<0.008). Room temperature storage of blood had no effects on MCH, regardless of diagnosis.
Biospecimens
Preservative Types
- None (Fresh)
Diagnoses:
- Other diagnoses
- Normal
Platform:
Analyte Technology Platform Cell count/volume Hematology/ auto analyzer Protein Hematology/ auto analyzer Protein Clinical chemistry/auto analyzer Pre-analytical Factors:
Classification Pre-analytical Factor Value(s) Storage Time at room temperature 0 days
1 day
2 days
3 days
4 days
5 days
6 days
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Study Purpose
The purpose of this study was to determine the effects of refrigerated storage of blood on hematological parameters. EDTA blood was obtained weekly from 8 healthy individuals for 3 weeks and stored at 6°C for up to 6 days.
Summary of Findings:
Hb, RBC count and MCH were stable during 6 days of refrigerated storage. Similarly to room temperature storage of blood, Ret-He declined slightly (<6%) over the 6 days of refrigerated storage. MCV increased slightly (5%), and RDW and reticulocyte counts declined slightly (<3% and <4%, respectively) when blood was refrigerated prior to analysis. Stability of all analytes was comparable to or improved when blood was refrigerated rather than stored at room temperature.
Biospecimens
Preservative Types
- Other Preservative
Diagnoses:
- Normal
Platform:
Analyte Technology Platform Cell count/volume Hematology/ auto analyzer Protein Clinical chemistry/auto analyzer Protein Hematology/ auto analyzer Pre-analytical Factors:
Classification Pre-analytical Factor Value(s) Storage Storage temperature Room temperature
6°C
Storage Storage duration 0 days
1 day
2 days
3 days
4 days
5 days
6 days