Impact of preanalytical variables on the analysis of biological fluids in proteomic studies.

Author(s): Ferguson RE, Hochstrasser DF, Banks RE

Publication: Proteomics Clin Appl, 2007, Vol. 1, Page 739-46

PubMed ID: 21136730 PubMed Review Paper? No

Purpose of Paper

Conclusion of Paper

Studies

1. Study Purpose

   The purpose of this study was to determine the effects of storage of serum or citrated plasma in contact with cellular components on the SELDI-TOF spectra. The temperature of post-centrifugation storage was unspecified, but based on authors recommendations, is assumed to be 18-20 degrees C.

   Summary of Findings:

   When serum was subjected to 4 h of storage in contact with cellular components after centrifugation rather than immediate separation, the SELDI-TOF spectra showed significant differences, particularly with respect to low molecular weight peptides. In comparison, smaller differences in the SELDI-TOF spectra of citrated-plasma were observed after post-centrifugation storage in contact with cellular components for 4 h rather than 0 h.

   Biospecimens

   • Bodily Fluid - Serum
   • Bodily Fluid - Plasma

   Preservative Types

   • None (Fresh)

   Diagnoses:

   • Not specified

   Platform:

   Analyte	Technology Platform
   Protein	SELDI-TOF MS

   Pre-analytical Factors:

   Classification	Pre-analytical Factor	Value(s)
   Storage	Storage duration	0 h 4 h
   Biospecimen Aliquots and Components	Blood and blood products	Plasma Serum

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