Efficient Method for the Proteomic Analysis of Fixed and Embedded Tissues

Author(s): Palmer-Toy Darryl, Krastins Bryan, Sarracino David, Nadol Joseph, Merchant Saumil

Publication: J Proteome Res, 2005, Vol. 4, Page 2404

PubMed ID: 16335994 PubMed Review Paper? No

Purpose of Paper

Conclusion of Paper

Studies

1. Study Purpose

   To validate a novel method of tryptic peptide release from formalin fixed paraffin embedded (FFPE) tissue specimens for peptide identification via liquid chromatography-mass spectrometry.

   Summary of Findings:

   A total of 573 peptides (FFPE, 442; frozen, 266 peptides) corresponding to 155 proteins were identified. Of these 40% and 20% were unique to FFPE and frozen tissue, respectively. Of the proteins identified, 35% and 12% were unique to FFPE and frozen tissues, respectively. A 24 h incubation in SDS during protein digestion yielded 116 identifiable proteins in comparison to the 573 proteins identified after a 1 h incubation.

   Biospecimens

   • Tissue - Ear

   Preservative Types

   • Formalin

   Diagnoses:

   • Normal

   Platform:

   Analyte	Technology Platform
   Peptide	GC-MS

   Pre-analytical Factors:

   Classification	Pre-analytical Factor	Value(s)
   Analyte Extraction and Purification	Protein digestion	1 h 24 h

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