Effect of blood collection tube types on the measurement of human epidermal growth factor.

Author(s): Yucel A, Karakus R, Cemalettin A

Publication: J Immunoassay Immunochem, 2007, Vol. 28, Page 47-60

PubMed ID: 17236396 PubMed Review Paper? No

Purpose of Paper

Conclusion of Paper

Studies

1. Study Purpose

   The purpose of this study was to evaluate whether blood EGF levels were influenced by the type of blood-storage tube. Blood taken in one draw was separated into six different tubes.

   Summary of Findings:

   EGF peptide levels were dependent upon the type of collection tube used; EGF levels were highest in polystyrene tubes, followed by glass, plastic with clot activator, plastic without clot activator, polypropylene, and plastic tubes with EDTA. EGF levels in blood separated sera differed based upon glass versus polypropylene collection tube, and polystyrene versus vac-gel, vac no-gel, and polypropylene tubes, but not glass versus polystyrene tubes. Plasma separated specimens collected in plastic tubes failed to generate detectable EGF levels. The authors hypothesize that the difference among EGF plasma and serum levels may be due to innate and continuing platelet activity during refrigerated storage.

   Biospecimens

   • Bodily Fluid - Plasma
   • Bodily Fluid - Serum
   • Bodily Fluid - Blood

   Preservative Types

   • None (Fresh)

   Diagnoses:

   • Normal

   Platform:

   Analyte	Technology Platform
   Peptide	ELISA

   Pre-analytical Factors:

   Classification	Pre-analytical Factor	Value(s)
   Biospecimen Acquisition	Type of collection container/solution	Round bottom glass tube Polystyrene tissue culture tube BD Vacutainer SST II Plus serum separation tube Plastic Vacuette Z-serum clot activator tube Plastic Vacuette K2E plasma separtion tube w/ EDTA Polypropylene with conical bottom
   Biospecimen Aliquots and Components	Blood and blood products	Plasma Serum

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2. Study Purpose

   The purpose of this study was to evaluate whether male and female patients had statistically significant differences in serum EGF levels.

   Summary of Findings:

   Female patients (17 subjects) exhibited statistically significantly higher EGF levels than male (17 subjects) patients. Both sexes displayed similar collection tube dependent trends in EGF levels.

   Biospecimens

   • Bodily Fluid - Serum
   • Bodily Fluid - Blood

   Preservative Types

   • None (Fresh)

   Diagnoses:

   • Normal

   Platform:

   Analyte	Technology Platform
   Peptide	ELISA

   Pre-analytical Factors:

   Classification	Pre-analytical Factor	Value(s)
   Preaquisition	Patient gender	Female Male

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