A simple technique for preservation of fixation-sensitive antigens in paraffin-embedded tissues.
Author(s): Beckstead JH
Publication: J Histochem Cytochem, 1994, Vol. 42, Page 1127-34
PubMed ID: 8027531 PubMed Review Paper? No
Purpose of Paper
Conclusion of Paper
Studies
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Study Purpose
The purpose of this study was to evaluate 13 different preservation techniques (routine formalin, zinc-formalin, paraformaldehyde, ethanol, commercial formalin alternative fixatives, and snap freezing) with regard to antigen and morphological preservation. Antibodies developed against the following antigens were investigated: CD1, CD4, CD7, CD8, CD19.
Summary of Findings:
The tissues preserved in zinc-based solutions without any formaldehyde content demonstrated excellent antigen and morphology preservation, closely approximating the number of cells stained and the intensity of staining in case-matched frozen sections, and the level of morphological detail observed with formalin-fixed paraffin-embedded sections. The other fixatives examined demonstrated little or no antigen survival. The authors identified 0.5% zinc chloride and 0.5% zinc acetate in Tris-Ca acetate buffer as the superior fixatives.
Biospecimens
Preservative Types
- Formalin
- Ethanol
- Frozen
- Other Preservative
Diagnoses:
- Not specified
Platform:
Analyte Technology Platform Protein Immunohistochemistry Morphology H-and-E microscopy Pre-analytical Factors:
Classification Pre-analytical Factor Value(s) Immunohistochemistry Specific Targeted peptide/protein CD1
CD4
CD7
CD8
CD19
Biospecimen Preservation Type of fixation/preservation B-5 fixative
Ethanol
Formalin (buffered)
Optimal Fix
Paraformaldehyde
Prefer
Quanta Fix
Snap frozen
Z-fix
Zinc acetate
Zinc chloride
Biospecimen Preservation Fixative additive/buffer None
Tris-Ca acetate buffer