Validation of tissue microarray technology in endometrioid cancer of the endometrium.

Author(s): Fons G, Hasibuan SM, van der Velden J, ten Kate FJ

Publication: J Clin Pathol, 2007, Vol. 60, Page 500-3

PubMed ID: 16822874 PubMed Review Paper? No

Purpose of Paper

Conclusion of Paper

Studies

1. Study Purpose

   The purpose of this study was to compare ER, PR, p53, and EMA IHC staining in TMAs versus whole section slides of endometrioid cancer specimens. The type of fixative used was not specified. The total percentage of positive cells after counting 2-3 cores, as well as the category (positive/negative) of staining, determined by the average percentage of positive cells per core, were compared to results from whole sections for ER and PR staining. For p53 and EMA IHC staining, staining was classified as negative, weakly, and strongly positive as opposed to individual percentages. Results from individual cores were added to get a final tumor score. For discrepant results among 3 cores from the same tumor, the staining category of the majority was used.

   Summary of Findings:

   A comparison of individual percentages of ER and PR positive cells resulted in complete concordance between TMAs and whole section slides for 47% and 40% of specimens, respectively, and results that differed by no more than 10% for 69% and 65% of specimens, respectively. While 26/38 cases showed positive ER staining on whole section slides, only 22/38 showed positive ER staining on TMA, resulting in agreement 90% of the time and an overall good correlation (K=0.78). All 4 discrepant cases were ER negative on TMA and ER positive on whole section slides. 35/40 cases showed positive PR staining on whole section slides, and 34/40 showed positive PR staining on TMA, resulting in agreement 92% of the time and an overall good correlation (K=0.96). Of the 3 discrepant cases, 2 were PR positive on whole section slides, but negative on TMA and 1 case was the other way around. For both ER and PR staining, discordant results were more common among specimens for which there were only 2 assessable cores, as opposed to 3. Complete concordance between TMAs and whole sections of p53 and EMA immunostaining was observed for 92% and 95% of specimens, respectively. For EMA, like ER and PR, concordance rates were higher for 3-core analysis (100%) than for 2-core analysis (79%), but 3-core analysis did not show higher correspondence than 2-core analysis for p53 immunostaining.

   Biospecimens

   • Tissue - Endometrium
   • Tissue - Spleen
   • Tissue - Liver

   Preservative Types

   • Other Preservative

   Diagnoses:

   • Normal
   • Neoplastic - Carcinoma

   Platform:

   Analyte	Technology Platform
   Protein	Immunohistochemistry
   Protein	Tissue microarray

   Pre-analytical Factors:

   Classification	Pre-analytical Factor	Value(s)
   Immunohistochemistry Specific	Targeted peptide/protein	ER PR p53 EMA
   Biospecimen Aliquots and Components	Type of slide	Tissue microarray Whole section
   Biospecimen Aliquots and Components	Aliquot size/volume	2 TMA cores 3 TMA cores

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