Use of paraffin wax embedded bone marrow trephine biopsy specimens as a source of archival DNA.

Author(s): Provan AB, Hodges E, Smith AG, Smith JL

Publication: J Clin Pathol, 1992, Vol. 45, Page 763-5

PubMed ID: 1401203 PubMed Review Paper? No

Purpose of Paper

Conclusion of Paper

Studies

1. Study Purpose

   The purpose of this study was to determine the effects of proteinase K digestion time on the extraction of DNA from FFPE bone-marrow trephine biopsies and lymph nodes.

   Summary of Findings:

   DNA yield and molecular weight increased with proteinase k digestion time. After 12 h, only low molecular weight DNA was obtained, but after 5 days, high molecular weight DNA was also obtained. The authors report no effect of specimen age on DNA yield or size. DNA was more reliably extracted from bone marrow trephine biopsies than lymph node specimens. HLA-DPB was successfully amplified from 6 of 10 trephine bone marrow biopsies, regardless of template dilution.

   Biospecimens

   • Tissue - Lymph Node
   • Tissue - Bone Marrow

   Preservative Types

   • Formalin

   Diagnoses:

   • Not specified

   Platform:

   Analyte	Technology Platform
   DNA	Electrophoresis
   DNA	PCR

   Pre-analytical Factors:

   Classification	Pre-analytical Factor	Value(s)
   Analyte Extraction and Purification	Protein digestion	Proteinase K at 37 degrees C
   Analyte Extraction and Purification	Incubation duration/condition	12 h 24 h 48 h 5 days
   PCR Specific	Template/input amount	Dilution of 1:10 Dilution of 1:20 Dilution of 1:50 Dilution of 1:100 Dilution of 1:200
   Biospecimen Acquisition	Biospecimen location	Bone marrow Lymph node
   Storage	Storage duration	0-5 years

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