Stability of complete blood count in different storage conditions using the ABX PENTRA 60 analyzer.
Author(s): Oliveira LR, Simionatto M, Cruz BR, Bittencourt JIM, Krum EA, Moss MF, Borato DCK
Publication: Int J Lab Hematol, 2018, Vol. 40, Page 359-365
PubMed ID: 29512875 PubMed Review Paper? No
Purpose of Paper
This paper investigated the effects of delayed analysis at room temperature or 4°C on hematological analytes in blood.
Conclusion of Paper
Delayed analysis resulted in significant changes in hematocrit, mean corpuscular volume (MCV), mean corpuscular hemoglobin concentration (MCHC), mean platelet volume (MPV), platelet count, and red cell distribution width (RDW) but the significance was dependent on storage temperature and duration. The mean coefficient of variance (CV) for MCHC, MPV, mean corpuscular hemoglobin (MCH), RDW, white blood cell (WBC) count, MCV, hematocrit, and platelet counts exceeded the acceptable range for the unspecified state-of-the-art method for some storage temperatures and durations. CVs higher than the maximum allowed for the ABX Pentra 60 were found after 24 h or more of storage at room temperature or 4°C for WBC count, platelet count, and MPV.
Studies
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Study Purpose
This study investigated the effects of delayed analysis at room temperature and 4°C on levels of hematological analytes in blood. Venous EDTA blood was obtained from 93 healthy patients (20-87 years old) and analyzed within 2 h of collection using a ABX Pentra 60 hematological analyzer. Specimens were stored at room temperature or 4°C and reanalyzed after 24 h, 48 h, and 72 h. Levels were compared to the baseline at 2 h and considered significant if P<0.05 using the Kruskal-Wallis analysis. CVs were compared to those of an unspecified state-of-the-art method and those recommended by the ABX Pentra 60 analyzer.
Summary of Findings:
Storage for 24 h at room temperature resulted in significant increases in the hematocrit and MCV and decreases in MCHC and platelets compared to baseline levels, but only MPV increased after 24 h at 4°C. In addition, significant increases in RDW were found after 48 h at room temperature and decreases in platelet count after 48 h at 4°C. No additional analytes were affected by storage for 72 h at either temperature. Further, CV’s higher than that acceptable for the unspecified state-of-the-art method were found after 24 h at room temperature or 4°C for MCHC, MPV, and platelet counts; after 24 h at room temperature for MCH, RDW, and WBC counts; after 48 h at room temperature for MCV; after 72 h at room temperature for hematocrit; and after 72 h at 4°C for MCV and RDW. CVs higher than the maximum allowed for the ABX Pentra 60 were found after 24 h or more of storage at room temperature or 4°C for WBC counts, platelets, and MPV.
Biospecimens
Preservative Types
- None (Fresh)
Diagnoses:
- Normal
Platform:
Analyte Technology Platform Cell count/volume Hematology/ auto analyzer Pre-analytical Factors:
Classification Pre-analytical Factor Value(s) Storage Storage temperature Room temperature
4°C
Storage Storage duration 2 h
24 h
48 h
72 h