Effect of tissue processing on the ability to recover nucleic acid from specific renal tissue compartments by laser capture microdissection.
Author(s): Tanji N, Ross MD, Cara A, Markowitz GS, Klotman PE, D'Agati VD
Publication: Exp Nephrol, 2001, Vol. 9, Page 229-34
PubMed ID: 11340308 PubMed Review Paper? No
Purpose of Paper
The purpose of this paper was to determine if amplifiable DNA could be successfully extracted from 1 to 4 laser capture microdissected (LCM) glomeruli or tubules.
Conclusion of Paper
Amplification of the beta-globulin gene was possible using DNA extracted from 1-4 LCM glomeruli or tubules.
Studies
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Study Purpose
The purpose of this study was to determine the effects of number of LCM glomeruli or tubules on the amplification of beta-globin from archived normal renal biopsies.
Summary of Findings:
Amplification of beta-globulin was possible using DNA extracted from 1-4 LCM glomeruli or tubules.
Biospecimens
Preservative Types
- Formalin
Diagnoses:
- Normal
Platform:
Analyte Technology Platform DNA PCR Pre-analytical Factors:
Classification Pre-analytical Factor Value(s) Biospecimen Aliquots and Components Aliquot size/volume 1 glomeruli
2 glomeruli
3 glomeruli
4 glomeruli
1 tubule
2 tubules
3 tubules
4 tubules
Biospecimen Acquisition Biospecimen location Glomeruli
Tubules
PCR Specific Targeted nucleic acid Beta-globin