Bicentric evaluation of stabilizing sampling tubes for assessment of monocyte HLA-DR expression in clinical samples.
Author(s): Hamada S, Jeannet R, Gossez M, Cour M, Argaud L, Francois B, Daix T, Venet F, Monneret G
Publication: Cytometry B Clin Cytom, 2021, Vol. , Page
PubMed ID: 34117826 PubMed Review Paper? No
Purpose of Paper
The purpose of this paper was to compare the stability of monocyte human leukocyte antigen-DR isotype (mHLA-DR) levels in EDTA and Cyto-Chex BCT blood specimens from patients with the novel 2019 coronavirus disease (COVID, COVID-19, COVID19) caused by the SARS-CoV-2 virus and healthy controls.
Conclusion of Paper
mHLA-DR levels increased with room temperature storage of blood in EDTA tubes from healthy donors and patients and were 2-fold higher than baseline (<1.5 h) after 48-72 h. Levels of mHLA-DR in blood from Cyto-Chex BCT were 20% lower than in the EDTA baseline specimens and were unaffected by up to 168 h of room temperature storage.
Studies
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Study Purpose
The purpose of this study was to compare the stability of mHLA-DR levels in EDTA and CytoChex blood specimens from patients with Covid-19 and healthy controls. Blood from 12 septic COVID-19 patients and six healthy donors was simultaneously collected into EDTA tubes and Cyto-Chex BCT. mHLA-DR was quantified within 1.5 h and again after 6, 24, 48, 72, and 168 h (BCT only) at room temperature. mHLA-DR quantification included staining with QuantiBrite HLA-DR/Monocyte mixture and lysis using flow cytometry.
Summary of Findings:
mHLA-DR levels increased with room temperature storage of blood in EDTA tubes from healthy donors or COVID-19 patients and were 2-fold higher than baseline (<1.5 h) after 48-72 h. Levels of mHLA-DR in blood from Cyto-Chex BCT were 20% lower than in the EDTA baseline specimens and were unaffected by up to 168 h of room temperature storage.
Biospecimens
Preservative Types
- None (Fresh)
Diagnoses:
- Normal
- Pneumonia/Respiratory Infection
Platform:
Analyte Technology Platform Protein Flow cytometry Pre-analytical Factors:
Classification Pre-analytical Factor Value(s) Preaquisition Diagnosis/ patient condition Healthy
Covid-19
Storage Storage conditions EDTA tube
Cyto-Chex BCT
Storage Storage duration <1.5 h
6 h
24 h
48-72 h
168 h
Biospecimen Acquisition Type of collection container/solution EDTA tubes
Cyto-Chex BCT