Stability of interleukin 6, soluble interleukin 6 receptor, interleukin 10 and CC16 in human serum.

Author(s): Kenis G, Teunissen C, De Jongh R, Bosmans E, Steinbusch H, Maes M

Publication: Cytokine, 2002, Vol. 19, Page 228-35

PubMed ID: 12393169 PubMed Review Paper? No

Purpose of Paper

Conclusion of Paper

Studies

1. Study Purpose

   The purpose of this study was to determine the effects of storing serum at 4-40 degrees C for up to 21 days and freeze-thaw cycling on the levels of IL-6, sIL-6R, IL-10 and uteroglobin. Arterial blood was drawn from post-operative patients was used for analysis of all 4 analytes and venous blood was drawn from healthy volunteers for sIL-6R and uteroglobin only. After experimental storage all specimens were stored at -20 degrees C until analysis.

   Summary of Findings:

   IL-6 concentrations were unaffected by storage of serum at 4, 20 or 30 degrees C for up to 21 days compared to storage at -20 degrees C for 21 days, but IL-6 levels declined progressively during storage of serum at 40 degrees C with a 12% decline noted at day 11. On the other hand, sIL-6R levels were similar in serum stored at 4 or -20 degrees C, but declined linearly with storage at 20, 30 or 40 degrees C reaching significance after storage for 21, 2, and 1 days, respectively. Similarly, IL-10 levels in serum were comparable in specimens stored at -20 or 4 degrees C, but were significantly lower than the -20 degrees C specimens when serum was stored at 20, 30 or 40 degrees C for 14, 7 and 1 days, respectively. Uteroglobin levels were comparable to levels in serum stored at -20 degrees C after storage of serum at 4, 20, 30 or 40 degrees C, regardless of storage duration. Levels of IL-6, sIL-6R, IL-10, and uteroglobin were unaffected by up to 4 freeze-thaw cycles.

   Biospecimens

   • Bodily Fluid - Serum

   Preservative Types

   • Frozen

   Diagnoses:

   • Normal
   • Other diagnoses

   Platform:

   Analyte	Technology Platform
   Protein	ELISA

   Pre-analytical Factors:

   Classification	Pre-analytical Factor	Value(s)
   Storage	Storage duration	1-21 days
   Storage	Freeze/thaw cycling	1 cycle 2 cycles 3 cycles 4 cycles
   Storage	Storage temperature	4 degrees C 20 degrees C 30 degrees C 40 degrees C
   ELISA Specific	Targeted peptide/protein	IL-6 sIL-6R IL-10 Uteroglobin
   Preaquisition	Diagnosis/ patient condition	Post-operative patient

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2. Study Purpose

   The purpose of this study was to determine the effects of clotting time and temperature on levels of IL-6, sIL-6R, IL-10, and uteroglobin in serum. Arterial blood was drawn from post-operative patients and venous blood was drawn from healthy volunteers. After experimental storage, all specimens were centrifuged, and serum was stored at -20 degrees C until analysis.

   Summary of Findings:

   There was no effect of storing blood for up to 4 days at room temperature or 4 degrees C, prior to centrifugation, on levels of IL-6, sIL-6R, IL-10, or uteroglobin in serum.

   Biospecimens

   • Bodily Fluid - Blood
   • Bodily Fluid - Serum

   Preservative Types

   • Frozen

   Diagnoses:

   • Normal
   • Other diagnoses

   Platform:

   Analyte	Technology Platform
   Protein	ELISA

   Pre-analytical Factors:

   Classification	Pre-analytical Factor	Value(s)
   Preaquisition	Diagnosis/ patient condition	.Post-operative patient
   Storage	Storage temperature	4 degrees C Room temperature
   Storage	Storage duration	30 min 1 day 2 days 4 days
   ELISA Specific	Targeted peptide/protein	IL-6 sIL-6R IL-10 Uteroglobin
   Biospecimen Aliquots and Components	Centrifugation	Centrifugation delays investigated

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