The use of chlorhexidine/n-propyl gallate as a urine preservative.

Author(s): Nillen JL, Smith SM

Publication: Clin Lab Sci, 2004, Vol. 17, Page 149-54

PubMed ID: 15314889 PubMed Review Paper? No

Purpose of Paper

Conclusion of Paper

Studies

1. Study Purpose

   The purpose of this study was to determine the effect of storage of urine with and without CPG at room temperature (0-1 y) on the stability of common urine analytes. Stability was determined by comparison with urine specimens stored at -70 in the absence of CPG.

   Summary of Findings:

   In the absence of preservative, urea nitrogen was the most stable (7 months) followed by potassium (5 months), sodium (3 months), total nitrogen (2 months), 3 methyl histadine (2 months) and chloride (28 days). In contrast, in the absence of CPG, pH, ammonium, creatinine and osmality changed rapidly, often within 24 h. The addition of CPG increased the stability of urea nitrogen (12 months), potassium (7 months), sodium (5 months), total nitrogen (3 months), 3 methyl histadine (3 months) and chloride (4 months). Ammonium which had been unstable without preservative, was stable for 14 d with CPG. pH was also stabilized by the addition of CPG. However, creatinine and osmality remained unstable, even after the addition of CPG. The authors conclude that CPG allows for storage of a urine specimen for at least 14 d at room temperature without a change in most urine analytes.

   Biospecimens

   • Bodily Fluid - Urine

   Preservative Types

   • None (Fresh)
   • Other Preservative
   • Frozen

   Diagnoses:

   • Not specified

   Platform:

   Analyte	Technology Platform
   Small molecule	Clinical chemistry/auto analyzer
   Electrolyte/Metal	Clinical chemistry/auto analyzer
   Small molecule	Ion exchange chromatography
   Gas	Chemiluminescence

   Pre-analytical Factors:

   Classification	Pre-analytical Factor	Value(s)
   Biospecimen Preservation	Type of fixation/preservation	Chlorhexadine/n-propyl gallate None (fresh) Frozen
   Storage	Storage duration	Less than 2 h 1d 14 d 28 d 2 months 3 months 4 months 5 months 6 months 7 months 8 months 9 months 10 months 11 months 1 year
   Storage	Storage temperature	-70 degrees C 21-25 degrees C

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