Effect of shipment, storage, anticoagulant, and cell separation on lymphocyte proliferation assays for human immunodeficiency virus-infected patients.
Author(s): Weinberg A, Betensky RA, Zhang L, Ray G
Publication: Clin Diagn Lab Immunol, 1998, Vol. 5, Page 804-7
PubMed ID: 9801338 PubMed Review Paper? No
Purpose of Paper
Conclusion of Paper
Studies
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Study Purpose
The purpose of this study was to evaluate the effects of anticoagulant, cell separation, transportation, cryopreservation and HIV status on lymphocyte proliferation in response to 4 viral stimuli.
Summary of Findings:
HIV positive specimens were more likely to undergo lymphocyte proliferation in response to stimulation independent of antigen used. A positive LPA result occurred 51% of the time when PBMC were used versus 17% of the time when whole blood was used, regardless of anticoagulant or overnight transportation. Further, the odds of a positive LPA result were higher from fresh specimens than from those shipped overnight. The use of heparin or citrate as anticoagulants increased the odds of positive LPA results compared to use of ACD. Furthermore, for 3 of 4 antigens, use of citrate tubes increased the odds of positive LPA results when compared with heparin in uninfected controls. For HIV infected patients, heparin and citrate performed similarly. The authors conclude that specimens should be collected in heparin or ACD tubes and separated on site for LPAs.
Biospecimens
Preservative Types
- None (Fresh)
- Frozen
Diagnoses:
- AIDS/HIV-related
- Normal
Platform:
Analyte Technology Platform Cell count/volume Radioassay Pre-analytical Factors:
Classification Pre-analytical Factor Value(s) Biospecimen Preservation Type of fixation/preservation Frozen
None (fresh)
Biospecimen Aliquots and Components Blood and blood products Peripheral blood mononuclear cells
Whole blood
Biospecimen Acquisition Anticoagulant Acid-citrate-dextrose
Heparin
Citrate
Preaquisition Diagnosis/ patient condition Previous tetanus vaccine
VZV positive
CMV unknown
CMV positive
HIV negative
HIV positive
Radioassay Specific Antigen Cytomegalovirus
Varicella-zoster virus
Candida
Tetanus
Toxoid antigens
Phytohemagglutinin
Storage Between site transportation method Mailed
Not transported