Comparison of BD Vacutainer SST Plus Tubes with BD SST II Plus Tubes for common analytes.

Author(s): Bush VJ, Janu MR, Bathur F, Wells A, Dasgupta A

Publication: Clin Chim Acta, 2001, Vol. 306, Page 139-43

PubMed ID: 11282105 PubMed Review Paper? No

Purpose of Paper

Conclusion of Paper

Studies

1. Study Purpose

   The purpose of this study was to determine the effects of collection tube type and storage at 4 degrees C on the gel for 43 different clinical chemistry analytes in serum. In some cases, blood was collected into the two types of tubes and analyzed, while in other cases, 1 mL of stored serum was added to the gel tubes prior to experimental storage and analysis. The authors do not state the temperature or duration of storage or the collection tube type for this set of specimens.

   Summary of Findings:

   Visual examination of the SST Plus and SST II gel tubes revealed similar appearance and barrier formation. 3/58 SST II tubes contained hemolysis compared to 1/58 SST Plus tubes. There were no clinically significant differences between tube types for any of the 43 analytes measured immediately, but estradiol, progesterone, and CA-125 decreased after storage of serum for 22-26 h (stored serum added to the tube with no clot) at 4 degrees C in either tube type, while the other analytes examined remained stable.

   Biospecimens

   • Bodily Fluid - Serum
   • Bodily Fluid - Blood

   Preservative Types

   • None (Fresh)

   Diagnoses:

   • Normal
   • Not specified

   Platform:

   Analyte	Technology Platform
   Carbohydrate	Clinical chemistry/auto analyzer
   Electrolyte/Metal	Clinical chemistry/auto analyzer
   Glycoprotein	Clinical chemistry/auto analyzer
   Lipid	Clinical chemistry/auto analyzer
   Morphology	Macroscopic observation
   Peptide	Clinical chemistry/auto analyzer
   Protein	Clinical chemistry/auto analyzer
   Small molecule	Clinical chemistry/auto analyzer
   Steroid	Clinical chemistry/auto analyzer

   Pre-analytical Factors:

   Classification	Pre-analytical Factor	Value(s)
   Biospecimen Acquisition	Type of collection container/solution	BD SST Plus BD SST II
   Storage	Type of storage container	BD SST Plus BD SST II
   Storage	Storage duration	<2 h 22-26 h

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