Influences of blood sample processing on low-molecular-weight proteome identified by surface-enhanced laser desorption/ionization mass spectrometry.

Author(s): Banks R E, Stanley A J, Cairns D A, Berrett J H, Clarke P, Thompson D, Selby P J

Publication: Clin Chem, 2005, Vol. 51, Page 1637

PubMed ID: 16002455 PubMed Review Paper? No

Purpose of Paper

Conclusion of Paper

Studies

1. Study Purpose

   The purpose of this study was to determine if the type of anticoagulant or the duration of time before centrifugation (0 -24 h at room temperature) impacts the protein spectral profile of plasma specimens.

   Summary of Findings:

   Compared to time zero controls, plasma samples demonstrated differential peaks in an anticoagulant dependent manner after a 4 hour delay at room temperature before processing; plasma specimens containing EDTA, heparin, and citrate displayed variation in 5-10%, 1-6%, and 0-3% of peaks, respectively, depending upon the chip used for analysis. A delay of 24 hours yielded even more robust changes, with 29% of peaks exhibiting either increased or decreased intensity compared to time zero controls.

   Biospecimens

   • Bodily Fluid - Plasma
   • Bodily Fluid - Blood

   Preservative Types

   • Frozen

   Diagnoses:

   • Normal

   Platform:

   Analyte	Technology Platform
   Protein	SELDI-TOF MS

   Pre-analytical Factors:

   Classification	Pre-analytical Factor	Value(s)
   Storage	Time at room temperature	30 min 1 h 2 h 4 h 24 h 0 h
   Biospecimen Acquisition	Anticoagulant	EDTA Citrate Heparin None
   SELDI-TOF MS Specific	Technology platform	CM10 chip IMAC30-Cu chip H50 chip
   Biospecimen Aliquots and Components	Centrifugation	Centrifugation delays investigated

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2. Study Purpose

   The purpose of this study was to determine if the type of collection tube or the duration of clotting time (0 to 24 h at room temperature) impacts the protein spectral profile of serum specimens.

   Summary of Findings:

   Compared to time zero controls, the spectral profile of serum samples collected in activator tubes showed significant changes in 12-36% of peaks after 4 hours dependent upon the chip used for analysis. However, many of these changes were increases in peak intensity and were present as soon as 30 minutes after venipuncture. Tube-dependent effects were limited to 2 peaks displaying a higher intensity in gel tubes, and 2 peaks displaying a lower intensity compared to activator tubes after 30 minutes at room temperature.

   Biospecimens

   • Bodily Fluid - Serum
   • Bodily Fluid - Blood

   Preservative Types

   • Frozen

   Diagnoses:

   • Normal

   Platform:

   Analyte	Technology Platform
   Protein	SELDI-TOF MS

   Pre-analytical Factors:

   Classification	Pre-analytical Factor	Value(s)
   Biospecimen Acquisition	Type of collection container/solution	Greiner vacuette activator Plain tube Gel serum separator
   Storage	Time at room temperature	0 min 30 min 1 h 2 h 4 h
   Biospecimen Aliquots and Components	Centrifugation	Centrifugation delays investigated

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