Recommended "order of draw" for collecting blood specimens into additive-containing tubes.

Author(s): Calam RR, Cooper MH

Publication: Clin Chem, 1982, Vol. 28, Page 1399

PubMed ID: 7074955 PubMed Review Paper? No

Purpose of Paper

Conclusion of Paper

Studies

1. Study Purpose

   The purpose of this study was to determine effects of anticoagulant and draw order on calcium and potassium levels in blood. Blood was collected from a single venipuncture, first into an SST, then a K3EDTA tube. After a second venipuncture in the same individual, the tube order used for collection of two specimens was reversed.

   Summary of Findings:

   When blood was collected first into the K3EDTA tube, levels of potassium were increased and calcium levels were decreased in the second collection which was drawn into an SST, but when the SST was used for the first collection and the K3EDTA tube was used for the second, no interference was observed. The authors note that similar interference can occur with other anticoagulants so a strict order of draw should be followed.

   Biospecimens

   • Bodily Fluid - Blood
   • Bodily Fluid - Serum
   • Bodily Fluid - Plasma

   Preservative Types

   • None (Fresh)

   Diagnoses:

   • Not specified

   Platform:

   Analyte	Technology Platform
   Electrolyte/Metal	Clinical chemistry/auto analyzer

   Pre-analytical Factors:

   Classification	Pre-analytical Factor	Value(s)
   Biospecimen Aliquots and Components	Aliquot sequential collection	1st collection 2nd collection
   Biospecimen Aliquots and Components	Blood and blood products	Plasma Serum

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