Effects of various pre-analytical conditions on blood-based biomarkers of Alzheimer's disease.
Author(s): Ladang A, Rigaud L, Sqalli G, Cavalier E
Publication: Clin Chem Lab Med, 2021, Vol. 59, Page e435-e437
PubMed ID: 34233385 PubMed Review Paper? No
Purpose of Paper
This paper compared levels of amyloid-β (Aβ) and total Tau (t-Tau) in plasma from blood of healthy men collected in K2EDTA Vacutainer, K3EDTA S-Monovette, and K2EDTA Microtainer MAP tubes that were subjected to 4 or 8 tube inversions and centrifuged immediately or after a 1 h delay in a rack or lying on the bench.
Conclusion of Paper
Aβ1-42, and Aβ1-40 levels were significantly higher when blood was collected in a K2EDTA Vacutainer rather than a K2EDTA Microtainer tube, but the ratio of Aβ1-42 to Aβ1-40 was unaffected by the type of blood collection tube. Levels of Aβ1-42, Aβ1-40 and t-Tau in plasma from K3EDTA S-Monovette and K2EDTA Microtainer tubes were comparable among the four processing conditions, but Aβ1-40 levels were higher in specimens collected in K2EDTA vacutainer tubes that were left lying on the bench for an hour before centrifugation than in those that were processed immediately.
Studies
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Study Purpose
This study compared levels of Aβ1-42, Aβ1-40 and t-Tau in plasma from blood of healthy men collected in K2EDTA Vacutainer, K3EDTA S-Monovette, and K2EDTA Microtainer MAP tubes that were subjected to 4 or 8 inversions and centrifuged immediately or after a 1 h delay (at room temperature) in a rack or lying on the bench. Blood was collected from seven healthy men (aged 20-35 years) into K2EDTA Vacutainer, K3EDTA S-Monovette, and K2EDTA Microtainer MAP tubes. One tube of each type was subjected to each of the following processing: i.) inverted 8 times, centrifuged (2860 g for 5 min) and aliquoted immediately; ii.) inverted 4 times, centrifuged (2860 g for 5 min), and aliquoted immediately; iii.) inverted 8 times, stored in rack at room temperature for 1 h, centrifuged (2860 g for 5 min) and aliquoted; and iv.) inverted 8 times, stored lying on the bench at room temperature for 1 h, centrifuged (2860 g for 5 min) and aliquoted. Plasma aliquots were stored frozen at -80°C for less than 15 days before analysis. Levels of Aβ1-42, Aβ1-40 and t-Tau in plasma were quantified with SiMoA technology using Neurology 3-Plex A on a SR-X platform analyzer. For each specimen, levels were quantified in two runs with one freeze-thaw cycle between runs.
Summary of Findings:
Mean Aβ1-42, Aβ1-40 and t-Tau levels were 15%, 13% and 16% higher, respectively, when blood was collected in K2EDTA Vacutainer rather than K2EDTA microtainer tubes, but the difference was only significant for Aβ1-42 (P=0.016) and Aβ1-40 (P=0.031). The ratio of Aβ1-42 to Aβ1-40 was unaffected by the type of blood collection tube. Levels of Aβ1-42, Aβ1-40 and t-Tau in plasma from K3EDTA S-Monovette and K2EDTA Microtainer tubes were comparable among the four processing conditions evaluated, but Aβ1-40 was 14-15% higher in specimens from K2EDTA Vacutainer tubes that were left lying on the bench for an hour (protocol iv) compared to those that were processed immediately (protocol i or ii).
Biospecimens
Preservative Types
- Frozen
Diagnoses:
- Normal
Platform:
Analyte Technology Platform Peptide Clinical chemistry/auto analyzer Protein Clinical chemistry/auto analyzer Pre-analytical Factors:
Classification Pre-analytical Factor Value(s) Biospecimen Acquisition Type of collection container/solution K2EDTA Vacutainer
K3EDTA S-Monovette
K2EDTA Microtainer MAP tubes
Biospecimen Aliquots and Components Biospecimen mixing Inverted 4 times
Inverted 8 times
Storage Storage conditions In rack
Lying on bench
Storage Time at room temperature 0 h
1 h
Biospecimen Aliquots and Components Centrifugation Centrifugation delays investigated