Measurement of serum and plasma osmolality in healthy young humans--influence of time and storage conditions.

Author(s): Seifarth CC, Miertschischk J, Hahn EG, Hensen J

Publication: Clin Chem Lab Med, 2004, Vol. 42, Page 927-32

PubMed ID: 15387444 PubMed Review Paper? No

Purpose of Paper

Conclusion of Paper

Studies

1. Study Purpose

   The purpose of this study was to determine the effects of storage, anticoagulant, thawing method and preservation with bacitracin and neomycin on the osmolality of serum and plasma.

   Summary of Findings:

   A significant increase in serum osmolality was observed in specimens stored at room temperature for 3 days or more or at 7 degrees C for 28 days or more. In contrast, storage at 7 degrees C for 1 day or -21 degrees C for 14 days or more resulted in decreased serum osmolality, and storage at -78 degrees C for up to 56 days did not affect serum osmolality. The osmolality of serum frozen at -78 degrees C was elevated when specimens were thawed for 12 h at 7 degrees C instead of for 5 min at 37 degrees C. The osmolality of lithium heparin-plasma decreased during storage at -21 degrees C and was not significantly different from that of serum. The authors report that K3EDTA-plasma showed changes similar to serum during storage but had higher initial osmolality. Adding bacitracin and neomycin slightly attenuated the effects of storing lithium heparin-plasma at -21 degrees C, but significant storage effects were still noted by day 14 (0=0.004).

   Biospecimens

   • Bodily Fluid - Serum
   • Bodily Fluid - Plasma
   • Bodily Fluid - Blood

   Preservative Types

   • None (Fresh)
   • Other Preservative
   • Frozen

   Diagnoses:

   • Normal

   Platform:

   Analyte	Technology Platform
   Small molecule	Clinical chemistry/auto analyzer

   Pre-analytical Factors:

   Classification	Pre-analytical Factor	Value(s)
   Storage	Storage duration	<60 min 1 days 3 days 14 days 28 days 56 days
   Storage	Storage temperature	-78 degrees C -21 degrees C 7 degrees C 22 degrees C
   Biospecimen Aliquots and Components	Blood and blood products	Plasma Serum
   Storage	Thaw temperature/condition	37 degrees C 7 degrees C
   Storage	Thaw duration	5 min 12 h
   Biospecimen Preservation	Type of fixation/preservation	Frozen None (fresh) Refrigeration
   Biospecimen Preservation	Fixative additive/buffer	Bacitracin Neomycin None
   Biospecimen Acquisition	Anticoagulant	Potassium EDTA Lithium heparin

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