Detection of chromosomal instability in paired breast surgery and ductal lavage specimens by interphase fluorescence in situ hybridization.

Author(s): King BL, Tsai SC, Gryga ME, D'Aquila TG, Seelig SA, Morrison LE, Jacobson KK, Legator MS, Ward DC, Rimm DL, Phillips RF

Publication: Clin Cancer Res, 2003, Vol. 9, Page 1509-16

PubMed ID: 12684427 PubMed Review Paper? No

Purpose of Paper

Conclusion of Paper

Studies

1. Study Purpose

   The purpose of this study was to determine the effects of analyzing air dried ductal lavage cells rather than methanol-fixed surgical touch preparations for morphological assessment and FISH analysis of malignant and benign breast tumors.

   Summary of Findings:

   Enough cells were obtained by ductal lavage for cytological and FISH evaluation in 34 and 32 of the 39 cases, respectively. Abnormal cells were found in ductal lavage specimens in 7 of 15 (47%) malignant cases and 4 of 19 (21%) benign cases. FISH analysis identified chromosomal abnormalities in 11 (79%) and 10 (71%) surgical touch preparations and ductal lavage specimens from malignant cases, respectively. Of the 11 benign cases, 1 showed chromosomal abnormalities by FISH in the ductal lavage and surgical touch preparation, and another showed chromosomal abnormalities by FISH only in the ductal lavage specimen. The authors concluded that ductal lavage specimens could detect genetic abnormalities associated with breast cancer but that FISH analysis was more specific and sensitive than cytology.

   Biospecimens

   • Cell - Breast Lavage

   Preservative Types

   • Other Preservative

   Diagnoses:

   • Neoplastic - Benign
   • Neoplastic - Carcinoma

   Platform:

   Analyte	Technology Platform
   DNA	FISH
   Morphology	Light microscopy

   Pre-analytical Factors:

   Classification	Pre-analytical Factor	Value(s)
   Biospecimen Preservation	Type of fixation/preservation	Air-dried Methanol
   FISH Specific	Targeted nucleic acid	Pericentromeric repetitive sequences of chromosome 1 Pericentromeric repetitive sequences of chromosome 8 Pericentromeric repetitive sequences of chromosome 11 Pericentromeric repetitive sequences of chromosome 17
   Preaquisition	Diagnosis/ patient condition	Infiltrating ductal carcinoma Infiltrating lobular carcinoma Ductal carcinoma in situ Lobular carcinoma in situ Atypical ductal hyperplasia Papillomavirus Hyperplasia
   Biospecimen Acquisition	Method of cell acquisition	Breast tumor touch preparation Ductal lavage/nipple aspiration

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